Sequences from confirmation amplicon


Confirmation sequences for line 647D06 and hit At3g59890 are shown.
Line ID 647D06
Locus At3g59890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22126357
Primers used for PCR CQ48 - 8474, show primer details
   
Sequence Name 10-K106018-0022-647-D06C-CQ48
GenBank Accession LN490661 [GenBank]
Sequencing primer gene specific primer, CQ48: 5'-AACAGACCCAAATGATGTAGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106018-0022-647-D06C-CQ48
TGCCGCTTGATGACGCTTTCCCCATTTTACCACTGCATCCATTCACCTAAAGTATAAAGA
TTAAAAAGAGTGAACGAATGGCTTATAACTTGATCCTACAATTACACTCTAGCAAAGATC
TATGTAACTTACCCTAAAATATTTCTGTTGCTAGAAAAAAAAAAAAGGGCAACGCTTTCC
CTTCTTAAATTTCCGGGGAAAACCCCAACCTTCATGGGAAAGGCAAAAGGCCCCACCCCA
AAAGGGTTGGGGAAGAAACCAAAACCCTGGATCAATTGCCCTTTCCCGGGCCCAAGGAAT
TTCCCGCTTCCCCCAAAAGTCCCCAATTTCCCCTTGGAAAAACCCCCCGAAAAACGGCTT
TCCCCTTGGCCAATAAAACTAACCCGGCCTTTAAACCCAAGGGAACCCTTTTATTTCTTT
CTAAAAAAAAACAAAAATGGGGGTTTTCCGGGAGGGGGCGGGATATTTCCATTTGAAAAG
GGCTCCTGGCCGGGAAAATCTCCTGGGACCCCCATGGATTTTGAGGGCCAATTGGGAAAA
AAAAAAAGAGTATTTCCCATTTTTTTTCCATTCCAAAAATGAAAATTCCCCCCCGTTTTT
AAA
   
Sequence Name 10-K106019-0022-647-D06C-8409
GenBank Accession LN490662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106019-0022-647-D06C-8409
GGACTGAGCCATTTACATTGAATATATCCTGCCCCGTCCTGTACATCCCCATTTCTCGTT
CTCTTCTAGAACGAATCAAATGGTTCCACTCGGCTTCAAAGGCCGTGTTAGTTTCATCGG
CAATGTGAAACGCTGTTTTCCGGTGGTATTGTCAATGGGAAAGTCGGAGACTTTCGAGGA
AGCTGGAAATTCCGTTGCGCCTGGTAACGGCATATCGATCATGGTATCTGATTACTTCCC
TAACACTCTTGAGTTGTGACATTTAGCATTTCCATTGATGATTGTGATTTTCCCGGAAAT
TTATAGATGGAAAGCTGTTGCCTTTTTTTTTTTTTCTACCACCAAAAATTTTTTGGGGAA
ATTTCCATAAATTTTTCCTAAAGGGAATTTGGGGGACCAATTTTAAACCCTTTCTTCCCC
CCTTTTAAACCTTTATCCTTAGGGGAAAGGGAGCCGGGGGAAAAAGGGGAAAACTTTTCT
TCAAAGGGGAAAATTCGGCGGGAGGAAATTTGGTCCCAACATCTTTGGGGTCTTTAAA
Primers used for PCR CQ49 - 8474, show primer details
   
Sequence Name 11-K106018-0022-647-D06C-CQ49
GenBank Accession LN491118 [GenBank]
Sequencing primer gene specific primer, CQ49: 5'-TGAAAGGGAAATTAAACGAATGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106018-0022-647-D06C-CQ49
ATTGGGTTCATTGCATTGATCCTAATATACCAAATTTATTGTTCATATTCAATTTTATAT
TTTTAGCTAGCTCCAACAAAAACAATGTTTCCGTACATTTTCGTTGTCAAAAAAACAAAA
GATGATTCATGACCTCTTAATTAAAGTGAAATCCGGACTCTGACCATTAATTAAGGAAAA
AGTAGAGAAACTCCGGTTTGATAAATTAATTAAATAGAAATTCCGGTTTGACCATTAAAA
TGAAGAGAAATTCCGGTTTTCAATTTTAGTAACCTTTCCGGTTTTGGTTGAAACACCCCA
ACCTTAACCAGAAGCTTTGGATCTGACGTAAAGGGAAAATGTCTAAAACCCTACCGTTCG
CCGGTTCGAATTTTTCAGACAAGGTTTGAACATAAATTCTAATTTCGTTGCTCGACAGCA
ATCTCGGACCAAGTGGAAACCGAAAATGGCGGCGAATGGGTACAGTTTCTAAACTGGATT
CACTCTCTTCCGACATTGGTTTGATGAATTGATCTTTAAAGTTGTCATTTTTGTTGTTGT
TCTTGTCTGGGATTTGATCACAGGCTAATGGCGGCATCTACATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 11-K106019-0022-647-D06C-OriL
GenBank Accession LN491119 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106019-0022-647-D06C-OriL
GTAGATGCCGCCATTAGCCTGTGATCAATCCCAGACAAGAACACCTTTAAAATGACAACT
TTAAAGATCAATTCATCAAACCAATGTTGGAAGAGAGTGAATCCAGTTTAGAAACTGTAC
CCATTCGCCGCCATTTTCGGTTTCCACTTGGTCCGAGATTGCTGTCGAGCAACGAAATTA
GAATTTATGTTCAAACCTTGTCTGAAAAATTCGAACCGGCGAACGGTAGGGTTTTAGACA
TTTTCCCTTTACGTCAGATCCAAAGCTTCTGGTTAAGGTTGGGGTGTTTCAACCAAAACC
GGAAAGGTTACTAAAATTGAAAACCGGAATTTCTCTTCATTTTAATGGTCAAACCGGAAT
TTCTATTTAATTAATTTATCAAACCGGAGTTTCTCTACTTTTTCCTTAATTAATGGTCAG
AGTCCGGATTTCACTTTAATTAAGAGGTCATGAATCATCTTTTGTTTTTTTGACAACGAA
AATGTACGGAAACATTGTTTTTGTTGGAGCTAGCTAAAAATATAAAATTGAATATGAACC
ATAAATTTGGTATATTAGGATCAATGCAATGAACCCAATGACCGACCTCAGGTAAGCAGC
AGATACATTGTTTTTTTCTCGTCATTCGTTTAATTTCCCTTTC

Last Updated on 06.12.2018 16:11