Sequences from confirmation amplicon


Confirmation sequences for line 648C02 and hit At4g05140 are shown.
Line ID 648C02
Locus At4g05140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2651512
Primers used for PCR 10ih - 8474, show primer details
   
Sequence Name 38-K112810-0022-648-C02C-10ih
GenBank Accession LN503040 [GenBank]
Sequencing primer gene specific primer, 10ih: 5'-TATCCAGACTGCTGATGATGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112810-0022-648-C02C-10ih
GTTGCTTGAAATGTAGATTATGTAGTGAATTTATTTCTCATCTATGTTTTGACACTATCC
ATTTTGCCCGGGTTCTTATACGAGAACACGGGACAACACGGGCTAGGCTCGTGGTATGCG
CTTGTCCTAATAGCAATGTACAATTGGTGGGATTTAGTTGGGAGGTACATACCGATGGTG
AAATGGCTAAATGTTGAGAACAGAAAAGGACTAACCGTCGCGGTTTTAACCCGGTTTCTC
CTCGTTCCGGCTTTCTACTTCACTGCAAAATATGGTGATCAAGGATGGCCTTTCTACTTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATTA
   
Sequence Name 13-K112809-0022-648-C02C-8409
GenBank Accession LN492114 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112809-0022-648-C02C-8409
ATTTCATTGAGTAGAAGGCCATCCTTGATCACCATATTTTGCAGTGAAGTAGAAAGCCGG
AACGAGGAGAAACCGGGTTAAAACCGCGACGGTTAGTCCTTTTCTGTTCTCAACATTTAG
CCATTTCACCATCGGTATGTACCTCCCAACTAAATCCCACCAATTGTACATTGCTATTAG
GACAAGCGCATACCACGAGCCTAGCCCGTGTTGTCCCGTGTTCTCGTATAAGAACCCGGG
CAAAATGGATAGTGTCAAAACATAGATGAGAAATAAATTCACTACATAATCTACATTTTC
AAGCAACAGCTCTTTGTTGTTTAGCCGTTTATCCTTGGAAACATCATCAGCANTCTGGAT
A

Last Updated on 06.12.2018 16:11