Sequences from confirmation amplicon


Confirmation sequences for line 648G02 and hit At3g29060 are shown.
Line ID 648G02
Locus At3g29060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11045117
Primers used for PCR CP39 - 8474, show primer details
   
Sequence Name 22-K031248-022-648-G02C-CP39
GenBank Accession LN495995 [GenBank]
Sequencing primer gene specific primer, CP39: 5'-CTCTAGTTTTGGTTCATCTTTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031248-022-648-G02C-CP39
AGTACTATATCATTTCTATAGTTTTGAAATTATATGTGAATAAGATAAGTATTGAATAAA
CTGTTCAAAGTATGAAACCCAATATACATTTGGTGTATTCATCATTAATTTGCTACATTC
ACAAAGATTAAACACCAAGAGATTAATTTAATTTGTTGAAAAAATTGAAAATAGTCAATT
TTCATATGATGTAGTTATGAAATTAATCCAAATTTAGCTGTTTTTTTTGTATTTGTTTTT
GGAGATGTTAAAATTTTATTTACACGCTCTTACTTCCTCCAAGTTCTTGAAAAGGCAAAG
GTACAGACTTGAAGGCTCTGTATTTCCCCACGTTGTTCAAATGCTCATTCTCCAACCTGA
TTACAAAAAAACAAAGATCATTAAAATTCATCATCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCA
   
Sequence Name 21-K031248-022-648-G02C-8409
GenBank Accession LN495549 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031248-022-648-G02C-8409
ATTTTATGATCTTTGTTTTTTTGTAATCAGGTTGGAGAATGAGCATTTGAACAACGTGGG
GAAATACAGAGCCTTCAAGTCTGTACCTTTGCCTTTTCAAGAACTTGGAGGAAGTAAGAG
CGTGTAAATAAAATTTTAACATCTCCAAAAACAAATACAAAAAAAACAGCTAAATTTGGA
TTAATTTCATAACTACATCATATGAAAATTGACTATTTTCAATTTTTTCAACAAATTAAA
TTAATCTCTTGGTGTTTAATCTTTGTGAATGTAGCAAATTAATGATGAATACACCAAATG
TATATTGGGTTTCATACTTTGAACAGTTTATTCAATACTTATCTTATTCACATATAATTT
CAAAACTATAGAAATGATAATAGTTACATCGCAATAACCAAGGAATGGTGTATGGCAAAG
ATGAACCAAAACTAGA

Last Updated on 06.12.2018 16:11