Sequences from confirmation amplicon


Confirmation sequences for line 649A11 and hit At3g21990 are shown.
Line ID 649A11
Locus At3g21990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7750279
Primers used for PCR CQ50 - 8474, show primer details
   
Sequence Name 34-K107033-0022-649-A11C-8409
GenBank Accession LN501436 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K107033-0022-649-A11C-8409
GGAATGAGCCATTTACAATTGAATATATATGAGTGTATACATTGAATATTAATATTTACA
AAGTGCTTTGAGTGTTTGGAAGGGATTTTGAGGAAGTTTCCAGAGTGTTGTGACGGTAAA
CAAGGAGGGAGAGTTTTGGGTACGAGCTGTAACTTTAGGTATGAGTTATATCCTTTTCTT
AGAAACTAACAACAACAGTACAATGAGAGCTCCATATTAAATGAAAGGGTTCTAGAGATA
AACAGTCTTATAATATACACTGTGTTGCATTTTAGTACGTGAACTTAATTGAGCGAATAA
ATGTACTTTCCTCTTTGGGATTTTTGTTTTTTTTTGTGAACTTGAGTTATTGAGAAGAAA
TGTTCAAGTATCTACTAGTAAATACATCCCACATAGAATACCAAATAGCTCAGCTCATAC
AAACTCTCTCATAATGAGAGAAAAAAAATCCTGAAATAAGGGTAAGAAAAACTCTTATGC
CTCAAGGTTTTAGTAAATCTCATAATTTGNGCCGAA
   
Sequence Name 42-K107034-0022-649-A11C-CQ50
GenBank Accession LN504478 [GenBank]
Sequencing primer gene specific primer, CQ50: 5'-GTTCGGCCAAAATTATGAGATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107034-0022-649-A11C-CQ50
TTTTCTTACCCTTATTTCAGGATTTTTTTTCTCTCATTATGAGAGAGTTTGTATGAGCTG
AGCTATTTGGTATTCTATGTGGGATGTATTTACTAGTAGATACTTGAACATTTCTTCTCA
ATAACTCAAGTTCACAAAAAAAAACAAAAATCCCAAAGAGGAAAGTACATTTATTCGCTC
AATTAAGTTCACGTACTAAAATGCAACACAGTGTATATTATAAGACTGTTTATCTCTAGA
ACCCTTTCATTTAATATGGAGCTCTCATTGTACTGTTGTTGTTAGTTTCTAAGAAAAGGA
TATAACTCATACCTAAAGTTACAGCTCGTACCCAAAACTCTCCCTCCTTGTTTACCGTCA
CAACACTCTGGAAACTTCCTCAAAATCCCTTCCAAACACTCAAAGCACTTTGTAAATATT
AATATTCAATTGTATACACTCATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT

Last Updated on 06.12.2018 16:11