Sequences from confirmation amplicon


Confirmation sequences for line 649C09 and hit At4g10510 are shown.
Line ID 649C09
Locus At4g10510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6496843
Primers used for PCR NB25 - 8474, show primer details
   
Sequence Name 74-K101221-0022-649-C09C-8409
GenBank Accession LN513032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K101221-0022-649-C09C-8409
CTTTTTGTTGCAGTGAGAGGAGTTAAAATCCTCTCCTGATTCACAGCCTCCTTTCCATGG
CTTGGTACCGGACCAATCTCGTTGTCGTTAAATACTTCAGATTCTGGCCATACTCCTATA
TTGACAAAACAAAGTTTAGTCATTGAGACGCTAAGACATATATAAGAACAGAAACTTAAG
AAATGTAAAAATATATATACCTGAGTCTATAATGCCAATGATCATCTGTTCACCCATATT
AGTTTGATTTAGAAGATTCTTTGGATTGGTGGGAGAAAGGCCTAAGTAGTCCCAAGTCCT
AGTTGTTGCCGGTTTGTAGAATCTATCCGGTATGACATGAACAACTTCAGGTAAATCTGT
TGCCAAAAAGCATTAGCAAAGAAAACAAAAACAGATTGTTCTGTTGAGACAATCAAGATG
GCCTTTAAATCTATGTTTCTGGTTCCTAAGAAGCTAAGAAAATTTGAGATTAGGATGGAG
TACCCGCTATTTTCTTGGCTTGGGACTCGGTAAGTTTGGCGGCGAAACCTGAGAAGCCAT
GTCGAANACTATGCACCATTGAACCATGGGCCTCTTCTTTACTGTAACATGAATGATGAA
GCANAAGCAAG
   
Sequence Name 74-K101228-0022-649-C09C-NB25
GenBank Accession LN513033 [GenBank]
Sequencing primer gene specific primer, NB25: 5'-GTTAATGGTTCTTGCTTTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K101228-0022-649-C09C-NB25
CCCATGGTTCATGGTGCATAGTTTTCGACATGGCTTCTCAGGTTTCGCCGCCAAACTTAC
CGAGTCCCAAGCCAAGAAAATAGCGGGTACTCCATCCTAATCTCAAATTTTCTTAGCTTC
TTAGGAACCAGAAACATAGATTTAAAGGCCATCTTGATTGTCTCAACAGAACAATCTGTT
TTTGTTTTCTTTGCTAATGCTTTTTGGCAACAGATTTACCTGAAGTTGTTCATGTCATAC
CGGATAGATTCTACAAACCGGCAACAACTAGGACTTGGGACTACTTAGGCCTTTCTCCCA
CCAATCCAAAGAATCTTCTAAATCAAACTAATATGGGTGAACAGATGATCATTGGCATTA
TAGACTCAGGTATATATATTTTTACATTTCTTAAGTTTCTGTTCTTATATATGTCTTAGC
GTCTCAATGACTAAACTTTGTTTTGTCAATATAGGAGTATGGCCAGAATCTGAAGTATTT
AACGACNACGAGATTGGTCCGGTACCAAGCCATTGGAAAGGAGGCTGTGAATCAGGAGAG
GATTTTAACTCCTCTCACTGCAACAAAAAGCTCATAGTGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
ANAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11