Sequences from confirmation amplicon


Confirmation sequences for line 649H06 and hit At3g06720 are shown.
Line ID 649H06
Locus At3g06720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2121559
Primers used for PCR 15mp - 8474, show primer details
   
Sequence Name 56-K114277-0022-649-H06C-15mp
GenBank Accession LN509177 [GenBank]
Sequencing primer gene specific primer, 15mp: 5'-GAATGATGGCTAAAAATCAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114277-0022-649-H06C-15mp
AGGGAATTATAAAATGGTACTGACAGGAGAAGTTCAACAAGTTTTGGGACAACACCGGCC
TGGATGACAGTCTGGATTTTGTCATTGGTCCCATCAGAGAGGTAAGAAAGAGCCCAACAG
GCATCTGTCAAGACTTCTTCATCATCTGAATGAATTAGTCGTTCAAGGGCGGGAAGAGCA
GGTTTGACCTGTTAAACATGCTTCATATGTTAGAAGAATACTAGACATAAACATTGACGA
AACAAGCTTGGAGGGGAAAAAAATAGACCTGGTCAAAGTGAGGCTGAGGCTTGCCACGAC
AGAAGTTTGACAAGGTCCAAGTGGCATTTCGAAGCATGGACAATTTAGCATGCTCATTAA
GCTGATTGAGCAGCGGAAGCAGTGCCCCACATCCAAGAACAAGATCACGGCACCGTGGTG
AATCACCAGCAACGATACACCATCAAGAACAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 26-K114276-0022-649-H06C-8409
GenBank Accession LN498031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114276-0022-649-H06C-8409
TACATTGATATATCCTGTTCTTGATGGTGTATCGTTGCTGGTGATTCACCACGGTGCCGT
GATCTTGTTCTTGGATGTGGGGCACTGCTTCCGCTGCTCAATCAGCTTAATGAGCATGCT
AAATTGTCCATGCTTCGAAATGCCACTTGGACCTTGTCAAACTTCTGTCGTGGCAAGCCT
CAGCCTCACTTTGACCAGGTCTATTTTTTTCCCCTCCAAGCTTGTTTCGTCAATGTTTAT
GTCTAGTATTCTTCTAACATATGAAGCATGTTTAACAGGTCAAACCTGCTCTTCCCGCCC
TTGAACGACTAATTCATTCAGATGATGAAGAAGTCTTGACAGATGCCTGTTGGGCTCTTT
CTTACCTCTCTGATGGGACCAATGACAAAATCCAGACTGTCATCCAGGCCGGTGTTGTCC
CAAAACTTGTTGAACTTCTCCTGTCAGTACCATTTTATAATTCCCTTATTTCATGTCCAC
TTCACACATTTGTTGACAAATAACTTGATACCTTGCTCTTGATTTT

Last Updated on 06.12.2018 16:11