SimpleSearch - Line and FST details
Line specific information
| Line ID | 649H06 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N462298) |
| Segregation Analysis | 50:50:34 |
| Confirmed for Hit | At3g06720 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 4332, 4423, 4536, 8425, 10028, 90599, 90600 |
| Note |
Gene hit At3g06720
| Sequence (A. th genome BLAST matches underlined) | >48-K023088-022-649-H06-8409 CCGGAATGAACCATTTACATTGAATATATCCTGATTCTTGATGGTGTATCGTTGCTGGTG ATTCACCACGGATGCCGTGATCTTGTTCTTGGATGTGGGGCACTGCTTCCGCTGCTCAAT CAGCTTAATGAGCATGCTAAATTGTCCATGCTTCAAAATGCCACTTGGACCTTGTCAAAC TTCTGTCGTGGCAAGCCTCAGCCTCACTTTGACCAGGTCTATTTTTTTCCCCTCCAAGCT TGTTTCGTCAATGTTTATGCCTATGGGATCCTCCCTATAGTGAGACTATGGAACCCCCCC TAAAATGACGCTAAATAAATCCAGACTAATTAGCACCTCTCCCTGATGAAAACACCCTTA TAACCATACAATATAAATAAT |
| GenBank Accession | BX660028 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:2121548 - go to primer design |
| BLAST e Value | 1e-95 |
| Hit Clone Code (BAC ID) | F3E22 |
| Hit Gene Code | At3g06720 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | importin alpha isoform 1 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At1g06950
| Sequence (A. th genome BLAST matches underlined) | >48-K023273-022-649-H06-8409 GAAGCCTTTACAATTGAATATATCCTGTTCCTGATGGTGTATCGCTGGTGATGACTCATC GCGCTCGTCTGTGAAACAATCCTACAATAAACTTAACAAATACTATGGGCTCCTCCCTAT AGTGAGAGGCACTGGGTTGGNANGCCCCATTTGGACGTTTGCGCCCAGCCGCTCTGCCCC CCTCCCCACCCTCCCTCGCACTGCGAGGGGCCGCCGCCCCCC |
| GenBank Accession | BX893710 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:2134428 - go to primer design |
| BLAST e Value | 2e-08 |
| Hit Clone Code (BAC ID) | F4H5 |
| Hit Gene Code | At1g06950 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | translocon at the inner envelope membrane of chloroplasts 110 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 561H09 |
Gene hit At3g06710
| Sequence (A. th genome BLAST matches underlined) | >48-K022837-022-649-H06-8409 TTGACAGGAAACCATATTTTTGCTCAGGAGGCAGGACAGTTTCTTTGTTATGAAGTGATA TCCCAAGGAAAACCACCTGACTATGGCTGGAAACGAGATTTGACGCACAGTGAGGAAAAT TCCTAAGTACGCAAAGGGAATTGACAGCGTCATCATGTATCCAAGAATGACAGGTGAGTT TTCGAATAAACCGCCACCTCCTACAATTGCTATTGCAATCCAGCGAAAAGAAAACGGCAG AAAAATAAAGAAGAAAGACACACATATGCTGATGCTAACAGCCTGTAGATAAGATAACCA CCACAACCTGCTATCCACAACATGGTACCATATGGCAACTAAACCACATAGGCTCAAACC GCCCCCCACCTCGACAAAAACCTCCCCCAAATACACGTCCCCCCCCCCCCCTCCACTCAC ACAACACAACATACCGAACACAA |
| GenBank Accession | BX660027 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:2119633 - go to primer design |
| BLAST e Value | 2e-152 |
| Hit Clone Code (BAC ID) | T8E24 |
| Hit Gene Code | At3g06710 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | E3 ubiquitin ligase |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | unknown |
|
Last Updated on 10.06.2021 13:37 |
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