Sequences from confirmation amplicon


Confirmation sequences for line 650B09 and hit At3g25170 are shown.
Line ID 650B09
Locus At3g25170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9165977
Primers used for PCR 73we - 8474, show primer details
   
Sequence Name 54-K118511-0022-650-B09C-73we
GenBank Accession LN508630 [GenBank]
Sequencing primer gene specific primer, 73we: 5'-TTGCTTGGAACACTAAATCCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118511-0022-650-B09C-73we
GACATATAGTGTATTTATGAGATGAGACATATAGGATTTTAGGAGACGAGTCTCGATGAA
ATTACTCAACAATAATAAGAGTCATACCATTATTATAAGCACAAAACATGGACAAAATTA
TTTCAGTTACAGGATCTTGTGTATTGAATTGAAATCAGTAAGATTTATATATTAATCAAC
TGGAATTATCATATACAGCGTAATGTGTATATATATGAATATATAATATCATTGCAAGTC
TCATGTAGAAGAAGGATCAATATAAAGGGACGTCGTCGTCATGTGAGGTTAAACCTACAT
GGATCAGCAATCTACATGGCACCGGGAAATAACATGGATCAGCAATGAGCAAAAGAAAAA
GAAATGAATTACCAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGATTATTAT
   
Sequence Name 26-K118510-0022-650-B09C-8409
GenBank Accession LN498137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118510-0022-650-B09C-8409
ATTGGTATTCATTTCTTTTTCTTTTGCTCATTGCTGATCCATGTTATTTCCCGGTGCCAT
GTAGATTGCTGATCCATGTAGGTTTAACCTCACATGACGACGACGTCCCTTTATATTGAT
CCTTCTTCTACATGAGACTTGCAATGATATTATATATTCATATATATACACATTACGCTG
TATATGATAATTCCAGTTGATTAATATATAAATCTTACTGATTTCAATTCAATACACAAG
ATCCTGTAACTGAAATAATTTTGTCCATGTTTTGTGCTTATAATAATGGTATGACTCTTA
TTATTGTTGAGTAATTTCATCGAGACTCGTCTCCTAAAATCCTATATGTCTCATCTCATA
AATACACTATATGTCTTATCTGACCTTGTCGTGAGTACGTAGAATCCAAAACCAAGAGGA
TTTAGTGTCCCAAGCA

Last Updated on 06.12.2018 16:11