Sequences from confirmation amplicon


Confirmation sequences for line 650C11 and hit At1g30720 are shown.
Line ID 650C11
Locus At1g30720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10899076
Primers used for PCR JV24 - 8474, show primer details
   
Sequence Name 71-K032929-022-650-C11C-8409
GenBank Accession LN512493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K032929-022-650-C11C-8409
GCCTTTACAATTGAATAATCCTGGTACGAAGCCTGGTGAGAATACGGTTTTAGCGACTTT
CATTGGGATGTATTTAGGCCGGTCGGATAAGCTGTTGACCGTGATGAACCGGGATTTCCC
GGAGTTGAAGCTGAAGAAAACCGATTGTACCGAGATGAGATGGATCGATTCGGTTCTGTT
TTGGGACGATTATCCGGTTGGTACACCGACTTCTGTGCTACTAAATCCGCTAGTCGCAAA
AAAGTTGTTCATGAAACGAAAATCGGACTACGTGAAGCGTCTGATTTCGAGAACCGATCT
CGGTTTGATACTCAAGAAATTGGTAGAGGTTGAGAAAGTTAAAATGAATTGGAATCCGTA
TGGAGGAAGGATGGGTGAGATCCCGAGTTCGAGGACACCATTCCCACATAGAGCAGGCAA
TTTGTTCAACATTGAGTATATCATAGACTGGTCAGAAGCTGGAGATAATGTGGAGAAGAT
ATTTTTTGGC
   
Sequence Name 71-K032929-022-650-C11C-JV24
GenBank Accession LN512494 [GenBank]
Sequencing primer gene specific primer, JV24: 5'-GTGCCAAATATTTCTTCTCCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K032929-022-650-C11C-JV24
CTCAATGTTGAACAAATTGCCTGCTCTATGTGGGAATGGTGTCCTCGAACTCGGGATCTC
ACCCATCCTTCCTCCATACGGATTCCAATTCATTTTAACTTTCTCAACCTCTACCAATTT
CTTGAGTATCAAACCGAGATCGGTTCTCGAAATCAGACGCTTCACGTAGTCCGATTTTCG
TTTCATGAACAACTTTTTTGCGACTAGCGGATTTAGTAGCACAGAAGTCGGTGTACCAAC
CGGATAATCGTCCCAAAACAGAACCGAATCGATCCATCTCATCTCGGTACAATCGGTTTT
CTTCAGCTTCAACTCCGGGAAATCCCGGTTCATCACGGTCAACAGCTTATCCGACCGGCC
TAAATACATCCCAATGAAAGTCGCTAAAACCGTATTCTCACCAGGCTTCGTACCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCA

Last Updated on 06.12.2018 16:11