Sequences from confirmation amplicon


Confirmation sequences for line 651F05 and hit At1g77660 are shown.
Line ID 651F05
Locus At1g77660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29186333
Primers used for PCR 55by - 8474, show primer details
   
Sequence Name 49-K108225-0022-651-F05C-55by
GenBank Accession LN506864 [GenBank]
Sequencing primer gene specific primer, 55by: 5'-CATGGTTTTGGAGTTTACTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K108225-0022-651-F05C-55by
GATGGTTTACGGTCAAGCCATGGCTTTGGAGTTCAACTTGTGCTGATGGAAGCTCTTTTG
TCGGAGAATTCAAATTTGGTGTCAAACATGGACTTGGTTCTTACCATTTCAGGTAATAAC
AAACAAAAGCTGTTATTTCTCTGTTTTATTTCTGAGTTCTGTTATTATGAAATTTTTGTT
GTTGTAGGAATGGAGATAAGTATGCAGGAGAGTATTTTGGAGACAAGATTCATGGATTTG
GTGTTTACCATTTCGCTAATGGTCACTATTACGAAGGAGCTTGGCATGAAGGTCGTAAAC
AAGGCTATGGCACGTATAGATTTAGAACCGGTGATATCAAATCCGGTGAATGGGATGATG
GTAATCTCGTTAACCATCTTCCTTTAGACTCTGACCCGGTTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 33-K108224-0022-651-F05C-8409
GenBank Accession LN501026 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K108224-0022-651-F05C-8409
AGCCATTTACAATTGAACCGGGTCAGAGTCTAAAGGAAGATGGTTAACGAGATTACCATC
ATCCCATTCACCGGATTTGATATCACCGGTTCTAAATCTATACGTGCCATAGCCTTGTTT
ACGACCTTCATGCCAAGCTCCTTCGTAATAGTGACCATTAGCGAAATGGTAAACACCAAA
TCCATGAATCTTGTCTCCAAAATACTCTCCTGCATACTTATCTCCATTCCTACAACAACA
AAAATTTCATAATAACAGAACTCAGAAATAAAACAGAGAAATAACAGCTTTTGTTTGTTA
TTACCTGAAATGGTAAGAACCAAGTCCATGTTTGACACCAAATTTGAATTCTCCGACAAA
AGAGCTTCCATCAGCACAAGTTTGAACTCCAAAGCCATGGCTTTGACCGTTAAACCATTC
ACCAGAGTAAGAATCTCCGGTGTAAAACCAGTAAACTCCAAAACCAT

Last Updated on 06.12.2018 16:11