Sequences from confirmation amplicon


Confirmation sequences for line 651G11 and hit At4g24610 are shown.
Line ID 651G11
Locus At4g24610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12702551
Primers used for PCR 20fx - 8474, show primer details
   
Sequence Name 78-K111632-0022-651-G11C-20fx
GenBank Accession LN513708 [GenBank]
Sequencing primer gene specific primer, 20fx: 5'-AACTTCTCAGCAAAACAAAACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K111632-0022-651-G11C-20fx
AGAAAATGCGGAAGTATGCTCCCTCATCCGGGACTACATATGGAATCACAGATCTTTTGG
TCAGTTTTTGGACATACTTGAAGCTTAGTTTTTTTGGAGCCAAGTTTTCTTTGAGAGGTG
ATAAGACATCTGCATACTGCTTCTCCAGAGCTTCCACTGTTGCCTTTTCAACATCAGCAA
TGGCCTAAAGAAACACAATCAGTGTCAGCCTTTTCACTCCAAATTCACACCATAAATACA
GTAAAGTTGTACGCTTATAACATGCAGTGGAATGTATACGCAATATATTCACAATCCCAA
AATCAAAAGTTATGAAGGAACAGCTAAACTTACACTCTCCAGAACAAAGATATATTCTGG
CCATCTGGAAATGATAACTTGATAGTCTTGAATGGTTTCGTTCAGCCTCTTGTACATTTC
ATCAACGAATGGAGTTGTTGAATGCTGTGTCCTGACTCCAGACCATTTTACCTGCTTGAA
TGACAGGTTTTGTAATGATCAAAGAAGTTAGTTTTAATGATACGACATAGGCTGAGAAGA
ACAAAGAGCAAGGGAAAATATAGAGAAAAAAAAATATCTGAAGAAAACCACACGGTAATT
ACCAGTCTTTACGGCGAGTTCTGTTACGCCTTTCATTTTATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 62-K111631-0022-651-G11C-8409
GenBank Accession LN510789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K111631-0022-651-G11C-8409
TTACAATTGAATTAAAATGAAAGGCGTAACAGAACTCGCCGTAAAGACTGGTAATTACCG
TGTGGTTTTCTTCAGATATTTTTTTTTCTCTATATTTTCCCTTGCTCTTTGTTCTTCTCA
GCCTATGTCGTATCATTAAAACTAACTTCTTTGATCATTACAAAACCTGTCATTCAAGCA
GGTAAAATGGTCTGGAGTCAGGACACAGCATTCAACAACTCCATTCGTTGATGAAATGTA
CAAGAGGCTGAACGAAACCATTCAAGACTATCAAGTTATCATTTCCAGATGGCCAGAATA
TATCTTTGTTCTGGAGAGTGTAAGTTTAGCTGTTCCTTCATAACTTTTGATTTTGGGATT
GTGAATATATTGCGTATACATTCCACTGCATGTTATAAGCGTACAACTTTACTGTATTTA
TGGTGTGAATTTGGAGTGAAAAGGCTGACACTGATTGTGTTTCTTTAGGCCATTGCTGAT
GTTGAAAAGGCAACAGTGGAAGCTCTGGAGAAGCAGTATGCAGATGTCTTATCACCTCTC
AAAGAAAACTTGGCTCCAAAAAAACTAAGCTTCAAGTATGTCCAAAAACTGACCAAAAGA
TCTGTGATTCCATATGTAGTCCCGGATGAGGTGAGCATAACTTTCCTGCATTTTTTTTCC
TTTACTTCTGTTAAAAAAATCGTTTTGTTTT

Last Updated on 06.12.2018 16:11