Sequences from confirmation amplicon


Confirmation sequences for line 652D01 and hit At2g19120 are shown.
Line ID 652D01
Locus At2g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8287753
Primers used for PCR 02um - 8474, show primer details
   
Sequence Name 34-K117356-0022-652-D01C-02um
GenBank Accession LN501574 [GenBank]
Sequencing primer gene specific primer, 02um: 5'-GCTGCAGCTTTTGCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117356-0022-652-D01C-02um
TCAGAGACCAGAAAGAGATGCATTGTTACAGCACCTTGCTGCGGTGGTTGAAGCCAGAGA
TAAAGTACTTGTTGAAATATCTCGCCTTCTTATTGTGGAGGGAAAGTTTCGTGCTGGTAA
CAATTTTAATCTTGAGGAAGCACGAGCGAGTCTTGAGGCAAGTTTTGCAAATGAGGCTGA
AATTGTTTTTACCACTGTATCAAGCAGTGGTCGTAAACTGTTTTCTCGTCTTACCCATGG
CTTCGATATGGTTGTCATTGACGAGGCTGCCCAAGCTAGTGAGGTTGGAGTTCTTCCTCC
CCTTGCTCTTGGTGCTGCGCGATGTGTACTTGTCGGTGATCCTCAGCAGCTTCCTGCGAC
AGTGATTAGCAAGGCTGCTGGAACTCTTTTATATAGTAGAAGTCTCTTTGAAAGGTTTCA
GCTAGCAGGCTGTCCGACTTTGTTATTGACTGTTCAATACAGAATGCATCCTCAAATTCG
AGATTTTCCTTCAAGGTACTTCTACCAAGGACGCCTCAAAGACAGTGAAAGTATATCCAG
CGCTCCTGATGAGATTTACTATAAAGATCCTGTTCTTAGGCGGGAGTATGAACAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTATATGTCCGCA
   
Sequence Name 14-K117355-0022-652-D01C-8409
GenBank Accession LN492688 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117355-0022-652-D01C-8409
TTATAGTAATCTCATCAGGAGCGCTGGATATACTTTCACTGTCTTTGAGGCGTCCTTGGT
AGAAGTACCTTGAAGGAAAATCTCGAATTTGAGGATGCATTCTGTATTGAACAGTCAATA
ACAAAGTCGGACAGCCTGCTAGCTGAAACCTTTCAAAGAGACTTCTACTATATAAAAGAG
TTCCAGCAGCCTTGCTAATCACTGTCGCAGGAAGCTGCTGAGGATCACCGACAAGTACAC
ATCGCGCAGCACCAAGAGCAAGGGGAGGAAGAACTCCAACCTCACTAGCTTGGGCAGCCT
CGTCAATGACAACCATATCGAAGCCATGGGTAAGACGAGAAAACAGTTTACGACCACTGC
TTGATACAGTGGTAAAAACAATTTCAGCCTCATTTGCAAAACTTGCCTCAAGACTCGCTC
GTGCTTCCTCAAGATTAAAATTGTTACCAGCACGAAACTTTCCCTCCACAATAAGAAGGC
GAGATATTTCAACAAGTACTTTATCTCTGGCTTCAACCACCGCAGCAAGGTGCTGTAACA
ATGCATCTCTTTTCTGGTCTCTTGAAATAAGGACTTCAGGATCAACTCCAACAGATCCCT
GAGAACGTGTAGCAAGGCCTGCAG

Last Updated on 06.12.2018 16:11