Sequences from confirmation amplicon


Confirmation sequences for line 652F02 and hit At2g19920 are shown.
Line ID 652F02
Locus At2g19920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8605919
Primers used for PCR HP51 - 8474, show primer details
   
Sequence Name 73-K033554-022-652-F02C-8409
GenBank Accession LN512835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K033554-022-652-F02C-8409
GCCATTTACATTGAATATATCCTCGACAAACCTCGACAACAAAGTCTAATTCCTCAGAAA
TTGCAGTGCAGCAAGCAGTTCAAAAGAATTTGCAGTATCCAACAAGCCTCTCTCCATGTC
TTTTGTCAATTCATTTAAGAAAAACTATGATAGATAGCACCAATATAGAACATAACAGAG
AACTGCCATACCATTCTTTCCTCGTTTTGGCCCAACTTGAACCACACTGCTCTTTCATAT
GCAACCATTGGCAAATCCTTCCATTGTCTGATCTCATCACCAGATATAACCTCACCCAGA
GATTTCCTGTTACAAAGACGATCACTTTCCAACGAAATAGATATAAAACTGATGGAAAAA
TCTCCAGCCTAAACATACCCTCCAAGATAGGTAAGCAACAAAAACGCCTTCTTGAATTCA
AGTTCACCCAAAGCTAATAGAAGAGAAATGTGCTTAGATTCTCCATTTTCATCTCCTCTC
AAGAACTTAGGAGACGGATCTTCACGATCAAGAGACACCTCTGCTACGAACAATCAACCA
TAGTCATAATATTG
   
Sequence Name 73-K033554-022-652-F02C-HP51
GenBank Accession LN512836 [GenBank]
Sequencing primer gene specific primer, HP51: 5'-ATAAAAGTGGAGACAAGACGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K033554-022-652-F02C-HP51
CTTTGGAACACTCANGAAGTTCTCAATATGCCGAATGTGAAGACCCTCGATGGGATAATC
ATATATTTTCTTAACGATGCGGTCACCGTTGATGGCTCTCCTCGCCTGTGGTCTGGCGAG
TCTCCAGTACAGTTTCCTAGAACTCCCGGGAAAAAAAGTTGCAGGGCTTCTCAAGGTCAG
TGTTAGGATAGAATCTGTTCACATACTTTGGGTTTTTTTTTTTCCTTCTTGTGTGTTTAG
AAGTGAAATTCTCTTCTTGTTGACCGCAATATTATGACTATGGTTGATTGTTTGTACCAA
AGGTGTCTCTTGATCGTGAAAATCCGTCTCCTAATTTCTTGAAAGGAAATAAAAATGGAA
AATCTAACCACATTTCTCTTCTATTAGCTTTGGGTGAACTTGAATTCAAAAAGGCGTTTT
TGTTGCTTACCTATCTGGAAGGGTATGTTTAGGCTGGAAATTTTTCCATCATTTTTATAT
CTATTTCGTTGGAAAGTGATCGTCTTTGTAACAGGAAATCTCTGGGTGAGGTTATATCTG
GTGATGAAATCAAACAATGGAAGGATTTGCCAATGGTTGCATATAAAAAACCATTGTGGT
TCAAGTTGGGCCAAAACAAGAAAAAAAGGGTATGGCAGTTCCCTGTTATGTTCAAAATGG
GGGCTACCAATCAAAGTTTTTCTTAAATGAATTGACAAAAAACATGGAAAGAGGCTTGTT
GAATACTGCAAATTCTTTTGAACTGCTTGCTGCACTGCAATTTCTAAGGAATTAAACTTT
GTTGTCAAGGTTTGTCAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CTTGGATCACCAATGAGTATGATGGTCAATATGAAAAAA
Primers used for PCR HP52 - 8474, show primer details
   
Sequence Name 74-K033554-022-652-F02C-HP52
GenBank Accession LN513007 [GenBank]
Sequencing primer gene specific primer, HP52: 5'-TAACGACGTAAACCAACCATGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K033554-022-652-F02C-HP52
AGTAGGGTTGTCACAGTATGTTGACTCCTTTGGTAATTGATCGAATCTAACGGTTAAAAC
ATTATCATCACCCAAAACCTTGTGCAGGTGTGTTCCAGTGTTCTCCAGAAAATAACCCTG
TAAAGACAGTCTTATCAATAATAAACATTATATCCTAAAAGTATCACTTGAATTCAGATC
ATCTTAAAACAAAGAACACGCATGACAGTGGGTGGAGAGCCGATTGGACCTTTAATCTGT
AGCTACCATCGGGAGCAACATGACATTGATAGTAATGAGTCTTCCCGCTATCCGATTCAA
GCTGCTGAGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAA
ATGG
   
Sequence Name 74-K033554-022-652-F02C-ORIL
GenBank Accession LN513008 [GenBank]
Sequencing primer gene specific primer, ORIL: unknown
Sequence (A. th genome BLAST matches underlined) 
>74-K033554-022-652-F02C-ORIL
TCGGATAGCGGGAAGATCATTACTATCAATGTCATGTTGCTCCCGATGGTAGCTACAGAT
TAAAGGTCCAATCGGCTCTCCACCCACTGTCATGCGTGTTCTTTGTTTTAAGATGATCTG
AATTCAAGTGATACTTTTAGGATATAATGTTTATTATTGATAAGACTGTCTTTACAGGGT
TATTTTCTGGAGAACACTGGAACACACCTGCACAAGGTTTTGGGTGATGATAATGTTTTA
ACCGTTAGATTCGATCAATTACCAAAGGAGTCAACATACTGTGACAACCCTTACTCTAAA
TACAAAGAGATTGCTAAGAATGGTATCATGGTTGGTTTACGTCGTT

Last Updated on 06.12.2018 16:11