Genotyping and primer details


Genotyping and primer details for 652F02 - At2g19920
Line ID 652F02
Locus At2g19920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8605919 of line 652F02 Chr2:8605919
   
Insertion allele amplicon 8474 - HP51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP51: 5'-ATAAAAGTGGAGACAAGACGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8606761
   
Insertion allele amplicon 8474 - HP52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP52: 5'-TAACGACGTAAACCAACCATGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8605524
   
wt amplicon (over InsPos) HP52 - HP51
wt amplicon start Chr2:8605524
wt amplicon end Chr2:8606761
wt amplicon size 1238 bp
Locus specific primer HP51: 5'-ATAAAAGTGGAGACAAGACGGAGA-3'
2nd locus specific primer HP52: 5'-TAACGACGTAAACCAACCATGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11