Sequences from confirmation amplicon


Confirmation sequences for line 653B01 and hit At2g30040 are shown.
Line ID 653B01
Locus At2g30040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12822802
Primers used for PCR RU83 - 8474, show primer details
   
Sequence Name 41-K027640-022-653-B01C-8409
GenBank Accession LN503948 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K027640-022-653-B01C-8409
ATCGGATGAGCCATTTCATTGAATATATCCTGACATGTAGATTCCATTTACATGAAGCCA
TTTACATGTAGATTTCCTTTTTTGCCTCTGACTCTTCGGAAGTGTCTCTAACCTCAGTCC
AACCATTAGATTCCCAATTTGCTCCTCCGGTTATAGCTAATTTACTTATCCTTGCCATCG
CCGAAACCATGGATTCTGGTCTCCATTCGTCGCTCTCTTCTTCTTCGTCAAACTCCGAGT
TGACCCAGTCAAGAACACAACGCGGAGACGACTCAGTGAAGAACGAGTCATGATGATCTT
GACACAGAAACGGATGCTGCAAGAGCTGATCACAGCTCCAACGCTGACTCCGATCACGTT
TCAAGCATTTCTCTAAGAAATCTCTCCCGAGTTCCGATAACCCCACCGGAATAAAAGGCA
AATCGTTAGAGAACCCGATCCGACTCAGCGAGTCGAAACCGTGATCTTCCCAAGCTGGTT
TTCCGGTGAGCATCTCGATGACGGTACACCCTAAAGACCACACGTCACTCTCCGGTCCTT
GGTAC
   
Sequence Name 42-K027640-022-653-B01C-RU83
GenBank Accession LN504331 [GenBank]
Sequencing primer gene specific primer, RU83: 5'-GGAGGTGGTTAGAAGAGAGTACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K027640-022-653-B01C-RU83
CGTCTCGAGATGCTCACCGGAAAACCAGCTTGGGAAGATCACGGTTTCGACTCGCTGAGT
CGGATCGGGTTCTCTAACGATTTGCCTTTTATTCCGGTGGGGTTATCGGAACTCGGGAGA
GATTTCTTAGAGAAATGCTTGAAACGTGATCGGAGTCAGCGTTGGAGCTGTGATCAGCTC
TTGCAGCATCCGTTTCTGTGTCAAGATCATCATGACTCGTTCTTCACTGAGTCGTCTCCG
CGTTGTGTTCTTGACTGGGTCAACTCGGAGTTTGACGAAGAAGAAGAGAGCGACGAATGG
AGACCAGAATCCATGGTTTCGGCGATGGCAAGGATAAGTAAATTAGCTATAACCGGAGGA
GCAAATTGGGAATCTAATGGTTGGACTGAGGTTAGAGACACTTCCGAAGAGTCAGAGGCA
AAAAAGGAAATCTACATGTAAATGGCTTCATGTAAATGGAATCTACATGTCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTG
TCT
Primers used for PCR 08ah - 8474, show primer details
   
Sequence Name 80-K118414-0022-653-B01C-08ah
GenBank Accession LN513995 [GenBank]
Sequencing primer gene specific primer, 08ah: 5'-CAACTTCACGTTGACACTAACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K118414-0022-653-B01C-08ah
TCTTAGTTCCTCTCTACGAACTTAAATATTTTACTCTATAATGTATGGTCCAATAAGAAG
CTATCAAACCAAAAACTGGGTGTGTTAAATGAGAGTTATCAACAAAAAAGGAGGTAACAA
TTGAGAGTTAAGGACACTCACGTAAAAGAGTTTTTATGTTAATAGATAGACCGATCCGAA
TCACATGCAATACCAAACAAAAAGTTAAGGCTGAGAATGAAAGATGTAGATTTCGAAAAA
TTATTCTTCTTATTATTGTTTTGATAATGATAATAACAACAACAACTACAACAATATAGA
ATATGCATAATAATACTACTGGTGTAAAACGTGGCATATATTTGAATATTCTCTACCACC
AATATCATCACCAACAATATTGCTTCACACGTCACTGCCGACGCCAACTCCGTCGCCGAC
TCTTCATTCCTCGGTTGTCTTACGGAATCATCGCTTGACGACTCCAATGATATGTAAGAT
TCCAATTCTACCCTTCAATTCTACATGTAAATGGCTTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 32-K118413-0022-653-B01C-8409
GenBank Accession LN500763 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118413-0022-653-B01C-8409
ATTTCATTGAATATATCCTGAAGCCATTTACATGTAGAATTGAAGGGTAGAATTGGAATC
TTACATATCATTGGAGTCGTCAAGCGATGATTCCGTAAGACAACCGAGGAATGAAGAGTC
GGCGACGGAGTTGGCGTCGGCAGTGACGTGTGAAGCAATATTGTTGGTGATGATATTGGT
GGTAGAGAATATTCAAATATATGCCACGTTTTACACCAGTAGTATTATTATGCATATTCT
ATATTGTTGTAGTTGTTGTTGTTATTATCATTATCAAAACAATAATAAGAAGAATAATTT
TTCGAAATCTACATCTTTCATTCTCAGCCTTAACTTTTTGTTTGGTATTGCATGTGATTC
GGATCGGTCTATCTATTAACATAAAAACTCTTTTACGTGAGTGTCCTTAACTCTCAATTG
TTACCTCCTTTTTTGTTGATAACTCTCATTTAACACACCCNGTTTTTGGTTTGATAGCTT
CTTATTGGACCATACATTATAGAGTAAAATATTTAAGTTCGTTAGAGAGGAACTAAGACC
ATGTAATTAAGTATAGTTAGGCTGATAAAGTATATGGTTAGTGTCACGTTGAAGTTG

Last Updated on 06.12.2018 16:11