Sequences from confirmation amplicon


Confirmation sequences for line 654A08 and hit At1g07550 are shown.
Line ID 654A08
Locus At1g07550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2323919
Primers used for PCR EU33 - 8474, show primer details
   
Sequence Name 07-K103431-0022-654-A08C-EU33
GenBank Accession LN489233 [GenBank]
Sequencing primer gene specific primer, EU33: 5'-AATATTTCTACACACCTATGGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103431-0022-654-A08C-EU33
GGCCGCTCAAACAGAACAACCAGGTTTTCCTGCAATAACAACCAAGTGGCTTAAGAAGTT
TGTTTGAAAGAGAACCTATAAATTGTTGTATCAAATTTTCATGTGTTTTACCTGAGAGAT
GCTCTTTTAAGTTTCCCTTTCCCATTAACTCGTAGATGAGAGCTAAGCCATTATCATCAT
CACAATATCCAATGAGGCTTACTAACTTTTCATGGTGAACTCTTAAAAGTAGTTCGACCT
AACAATCAAGGACGACATAAAGAAAAAAGTGTAACCAATTGATTCCTGATCTCAAACATT
AACCTCTTTTTCCAAAACTTTAGTAAAAGATTTAATAAGAATACCTCTGTTTTGAATTCT
TTATAGCCCTGAGCTGATGAATGAGAAAGCACTTTAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
ATA
   
Sequence Name 23-K103432-0022-654-A08C-8409
GenBank Accession LN496518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103432-0022-654-A08C-8409
AGAATTCAAACAGAGGTATTCTTATTAAATCTTTTACTAAAGTTTTGGAAAAAGAGGTTA
ATGTTTGAGATCAGGAATCAATTGGTTACACTTTTTTCTTTATGTCGTCCTTGATTGTTA
GGTCGAACTACTTTTAAGAGTTCACCATGAAAAGTTAGTAAGCCTCATTGGATATTGTGA
TGATGATAATGGCTTAGCTCTCATCTACGAGTTAATGGGAAAGGGAAACTTAAAAGAGCA
TCTCTCAGGTAAAACACATGAAAATTTGATACAACAATTTATAGGTTCTCTTTCAAACAA
ACTTCTTAAGCCACTTGGTTGTTATTGCAGGAAAACCTGGTTGTTCTGTTTTGAGCTGGC
CGATAAGACTTAAAATAGCTCTTGAATCCGCCATAGGTGTGTAGAAATATT
Primers used for PCR SP09 - CR3S, show primer details
   
Sequence Name 41-K117832-0022-654-A08C-SP09
GenBank Accession LN504242 [GenBank]
Sequencing primer gene specific primer, SP09: 5'-CTTAAGTGGAAACAAACTTAGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117832-0022-654-A08C-SP09
AAGGAAGGATTAAAGCTATTGTAAGATTTCTTTTTGAGATGATCTTTATCGATTTACTAC
GTGCCCACTCTCGTTTCTGAAATTCACTACGTTCTTTATTCTTTAGGGTTGACGAAAATA
TGATTTGTGTGTCTTGCGGTACAAGATTCCCAACAGCTGCGGTTGCGGCATCTGTTTCTG
CAGTGGCCATTATCATACTTGTGTTAGTTCTCATATTTGTTTTAAGAAGGAGAAAGCCAT
CTGCTGGGAAAGGTACTTAAAGGAGAATAACATATAGAAGAATATAATCCCATATTGTAT
AGCTCTCATGGAATTCTGTTAAATTTTTCTATGCAGTTACACGCTCATCCTTCAAATCGG
AAAATAGAAGATTTACTTACTCTGACGTTAACAAAATGACAAATAACTTTCAAGTTGTTA
TTGGTAAAGAAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTA
AGGGTTTCTTAAATGCTCA
   
Sequence Name 13-K117831w-0022-654-A08C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117831w-0022-654-A08C-CR3S
TAAATTTCTAATTTCTTTACCATAACAACTTGAAAGTTATTTGTCATTTTGTTAACGTCA
GAGTAAGTAAATCTTCTATTTTCCGATTTGAAGGATGAGCGTGTAACTGCATAGAAAAAT
TTAACAGAATTCCATGAGAGCTATACAATATGGGATTATATTCTTCTATATGTTATTCTC
CTTTAAGTACCTTTCCCAGCAGATGGCTTTCTCCTTCTTAAAACAAATATGAGAACTAAC
ACAAGTATGATAATGGCCACTGCAGAAACAGATGCCGCAACCGCAGCTGTTGGGAATCTT
GTACCGCAAGACACACAAATCATATTTTCGTCAACCCTAAAGAATAAAGAACGTAGTGAA
TTTCAGAAACGAGAGTGGGCACGTAGTAAATCAATAAAGATCATCTCAAAAAGAAATCTT
ACAATAGCTTTAATCCTTCCTTTTTTCTATCAAGAAGGGCTTGAGGAACCAAACCGCTCA
GTTTGTTCCCACTTAA

Last Updated on 06.12.2018 16:11