Sequences from confirmation amplicon


Confirmation sequences for line 654D08 and hit At3g57720 are shown.
Line ID 654D08
Locus At3g57720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21388780
Primers used for PCR KF34 - 8474, show primer details
   
Sequence Name 19-K103250-0022-654-D08C-8409
GenBank Accession LN494721 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103250-0022-654-D08C-8409
GATCACCCCTTTAAGTGTTGTCATCTCATCAGCTCACATCATCTGAGCGCCTACACCACT
CACGCTTCCACATGCACTTTTCAAGCATTTGTAAGGTCTTCAATCACCTTAGGATCGATT
ACTCCATCAAGCTGCCCATTCTCATGAAAGCCTTTCACGTAGCTAGCAATATGTTTGTAG
CCCCCATCAGAGCTAGTGGATGCAAGTGCTGGTTTACCAGTGAGAATAACCATCAAGAAG
ACTCCAAAGCTGTACACGTCGGTATATTCCGTCACCATTTTCCTGGTATAATATACTGGA
TCAAGGTACCCGATTGTTCCCTGAACACATTCAACTTCTATTCTTGATTTTCCCTCAGGG
AGGTTTATAGATATAGAGAAATCAGACAACTTTGGGGTCCAATTCTCGTCCAAGAAAACA
TTCCTTGGCTTAATGTGTCTATGTATGAAGATCTTGGGGAATGCCATGTGAAGATAAGTC
ACAGCATTTGCAATCTCCTTTCCAATCTTCAACCTTAAACTCAAAGGCAATATAACTTCC
TCCCCATTGACTATAACACCTCCTCTGTGATTCAAAACTCCATGTTCTGCATATTCAAAC
ACAAGAACCCGGAAG
   
Sequence Name 67-K103251-0022-654-D08C-KF34
GenBank Accession LN511792 [GenBank]
Sequencing primer gene specific primer, KF34: 5'-TGTCTTGAGTTTAGTCTTCCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103251-0022-654-D08C-KF34
TTGATCCAGAGGAGGTGTTATAGTCAATGGGGAGGAAGTTATATTGCCTTTGAGTTTAAG
GTTGAAGATTGGAAAGGAGATTGCAAATGCTGTGACTTATCTTCACATGGCATTCCCCAA
GATCCTCATACATAGACACATTAAGCCAAGGAATGTTTTCTTGGACGAGAATTGGACCCC
AAAGTTGTCTGATTTCTCTATCTCTATAAACCTCCCTGAGGGAAAATCAAGAATAGAAGT
TGAATGTGTTCAGGGAACAATCGGGTACCTTGATCCAGTATATTATACCACGAAAATGGT
GACGGAATATACCGACGTGTACAGCTTTGGAGTCTTCTTGATGGTTATTCTCACTGGTAA
ACCAGCACTTGCATCCACTAGCTCTGATGGGGACTACAAACATATTGCTAGCTACGTGAA
AGGCTTTCATGAGAATGGGCAGCTTGATGGAGTAATAGATCCTAAGGTGATGGAAGACAT
TACAAGTGCTCAAAAAGTGCATGTGGAAGCGTGTGTGGTGTTGGCGCTGAGATGCTGCGA
GCTGAGAGATGAAAACAGACCAAAGATGATCCAAATAGCTAAAGAACTCAAGCAGATTGA
GACATTATTTCGGAGATCATAGCTACAGATCCTCATGTAGTGAAAGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCG

Last Updated on 06.12.2018 16:11