Sequences from confirmation amplicon


Confirmation sequences for line 654H10 and hit T14K23 are shown.
Line ID 654H10
Locus T14K23 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14498220
Primers used for PCR KG46 - 8474, show primer details
   
Sequence Name 01-K038030-022-654-H10C-8409
GenBank Accession LN486488 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K038030-022-654-H10C-8409
GCCATTTCAATTGAATATATCCTGCCGGAGATGAAGAATCTTACAGATATCCTAACAGGT
GGCGATCTACTAAATCATCTATAACCCTAATGAGAAACCCTAACCCTAACAAGTGGATTA
CTCAGACATGATCATAGAAGAACAAAACATGTTTGAATAAGAAATCAATAATGAATATAA
CAAGAGAAAAGAGTTTAGAAGGATCTTCTCTTAAGGGAGTCTCCACAGGATTTTACACCC
AAGATTAGAAGAGAAAAATAGAATAGCCTCCCAAAACCTTAGGTCTAAACAATGACCTTA
AAACTATATATATAAGTTTCTAAAACGTGATGGGTTTTCTTTAAAAATAAGGGAAAGTTC
AGGGCTGAATCTGCAAATCTTCATAACTTCAATGAAACGCGAAATTGAATTGCCATCAGA
TATTGGTGTCGCTCGACACCAGAGGTTGTATCGATCGACACCTACGTGATTTTTGGTCTC
TGGATTTCATCGCGGTATACGTTCTCTGTTTTTCATTGGGATGCTC
   
Sequence Name 57-K038030-022-654-H10C-KG46
GenBank Accession LN509348 [GenBank]
Sequencing primer gene specific primer, KG46: 5'-GGAGCATCCTAATGAAAAACAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K038030-022-654-H10C-KG46
AAAAATCACGTAGGTGTCGATCGATACAACCTCTGGTGTCGAGCGACACCAATATCTGAT
GGCAATTCAATTTCGCGTTTCATTGAAGTTATGAAGATTTGCAGATTCAGCCCTGAACTT
TCCCTTATTTTTAAAGAAAACCCATCACGTTTTAGAAACTTATATATATAGTTTTAAGGT
CATTGTTTAGACCTAAGGTTTTGGGAGGCTATTCTATTTTTCTCTTCTAATCTTGGGTGT
AAAATCCTGTGGAGACTCCCTTAAGAGAAGATCCTTCTAAACTCTTTTCTCTTGTTATAT
TCATTATTGATTTCTTATTCAAACATGTTTTGTTCTTCTATGATCATGTCTGAGTAATCC
ACTTGTTAGGGTTAGGGTTTCTCATTAGGGTTATAGATGATTTAGTAGATCGCCACCTGT
TAGGATATCTGTAAGATTCTTCATCTCCGGCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11