SimpleSearch - Line and FST details
Line specific information
| Line ID | 654H10 |
| Vector Used | pGABI1 |
| Line Availability | available as T3 set from NASC (N462782) |
| Segregation Analysis | 50:50:40 |
| Confirmed for Hit | T14K23 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 4136, 4256, 7241, 9874, 93421, 93428, 93429, 93430 |
| Note |
Gene hit At5g63580
| Sequence (A. th genome BLAST matches underlined) | >80-K021385-022-654-H10-8409 GNAGCCATTTACATTGAATATATCCTGGAAGCCGTTAAAAATTGAGTAGCTGAATGCATC CTTGGTCAACACTGTATATATACGGCTATGTTTGTGCATAATAGTTCCGTATTGTAAAAG TTAACTCTTGCTGCAAACATCATGCTGCGTCAAATTCGGGAGTACAACATTCACTCTCCG TGATGTATGAGACTATCTCGATATGAAGAACCTCCTGCATAGGAGAGCATCACAAGTTTT ATCCCCTTTAAAAAAAAAAATGAAATAGTGGCTTCTCCAATTTTCACATGACCACCCACT GACTTTAGATTAAGTGACAATGGTTCCACAAAAGTGGTGAGTACTCTATTTGATAAAAGG GCAGTTCTTGGCAGATGCCGCAATGCCTCTCATATTCATTTGTAAACACGAGTTTGCATG GACCTCATACATTGGCGTTTTTTTCTGTTAATCCAATGATCCAAACATATGGTATGGGAT CCTCCCTATAGTGAG |
| GenBank Accession | BX660511 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:25456393 - go to primer design |
| BLAST e Value | 6e-08 |
| Hit Clone Code (BAC ID) | MLE2 |
| Hit Gene Code | At5g63580 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | flavonol synthase 2 |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 654F09 |
Gene hit T14K23
| Sequence (A. th genome BLAST matches underlined) | >80-K021351-022-654-H10-8409 CGGAATGAGCCTTTACAATTGAATATATCCTGAATCATGAAGAATATGAAGATACAGATG CGGCGGGATCAAAGTGGAGTTGATGGCTTGATTGTTCAAGATTGTTCCGTAACAACGAGA GTATCCCTTGTTGTAGCCATCAATCTGCTTCATCAATGGGAATTCTAGAGGAAGTAACCG TGAAGTATGGGATCCTCCCTATAATGAGGATCTTCTCTTAAGGGAGTCTCCACAGGATTT TACACCCAAGATTAGAAGAGAAAAATAGAATAGCCTCCCAAAACCTTAGGTCTAAACAAT GACCTTAAAACTATATATATAAGTTTCTAAAACGTGATGGGTTTTCTTTAAAAATAAGGG AAAGTTCAGGGCTGAATCTGCAAATCTTCATAACTTCAATGAAACGCGAAATTGAATTGC CATCAGATATTGGTG |
| GenBank Accession | BX660510 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:14498211 - go to primer design |
| BLAST e Value | 1e-126 |
| Hit Clone Code (BAC ID) | T14K23 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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