Sequences from confirmation amplicon


Confirmation sequences for line 655E05 and hit At1g68130 are shown.
Line ID 655E05
Locus At1g68130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25533709
Primers used for PCR MN45 - 8474, show primer details
   
Sequence Name 15-K031753-022-655-E05C-8409
GenBank Accession LN492855 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031753-022-655-E05C-8409
GAGCCATTTACAATTGAATATACCCGGGGGACATGAGCAGCTACACAAACCGCTTCAACC
GCGGAAAACAACGAGAACGGGGACCTCTCCATTGGTCCTATATTGCCTGGACATCCTTTA
CAAAGAAGACAATCCCCACCGTCGGAACAACAACCATCCACTTTGCTCTATCCCTTCGTT
ACTAATGGTAGTATCGAGCTTCAGCTACTTCCATCGAGGAATTGTGCTGATGAGACCAGC
CTTAGTCTGTCTATAGGGACAATGGATCAAAAGACAATGTCGGAAGTTGAGAAGAAGAGC
TACGAGAAGGGAGAAACGAGCCTAGAAAGAGAGGAGGCGAGAAGAGAAACAAAGAGGCAG
ATCGAAATCGCGGAATTGGAGTTTGCTGAAGCCAAGAGAATAAGGCAACATGCGAGAGCT
GAGCTTCACAAAGCTCATCTTTTTAGAGAAGAAGCAAGTAGGAGGATTAGTGCA
   
Sequence Name 16-K031753-022-655-E05C-MN45
GenBank Accession LN493322 [GenBank]
Sequencing primer gene specific primer, MN45: 5'-TTGCACTAATCCTCCTACTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031753-022-655-E05C-MN45
GCTCTCGCATGTTGCCTTATTCTCTTGGCTTCGCAAACTCCAATTCCGCGATTTCGATCT
GCCTCTTTGTTTCTCTTCTCGCCTCCTCTCTTTCTAGGCTCGTTTCTCCCTTCTCGTAGC
TCTTCTTCTCAACTTCCGACATTGTCTTTTGATCCATTGTCCCTATAGACAGACTAAGGC
TGGTCTCATCAGCACAATTCCTCGATGGAAGTAGCTGAAGCTCGATACTACCATTAGTAA
CGAAGGGATAGAGCAAAGTGGATGGTTGTTGTTCCGACGGTGGGGATTGTCTTCTTTGTA
AAGGATGTCCAGGCAATATAGGACCAATGGAGAGGTCCCCGTTCTCGTTGTTTTCCGCGG
TTGAAGCGGTTTGTGTAGCTGCTCATGTCCCCGGGTATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATG
G

Last Updated on 06.12.2018 16:11