Sequences from confirmation amplicon


Confirmation sequences for line 655G04 and hit At5g40350 are shown.
Line ID 655G04
Locus At5g40350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16139692
Primers used for PCR JV41 - 8474, show primer details
   
Sequence Name 30-K101325-0022-655-G04C-8409
GenBank Accession LN499645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101325-0022-655-G04C-8409
TCGGAATGAGCCTTTACATTGGTGGGTATAACAAAGCTTTTCACTCTTTTCAGCCATTTG
TTTGAGTTTTAAACACGTTTCGAAATTCCCTCACCACTAACATTAAAAAACCTCGTGCCG
ATTCTACCACAACTTGTGCATGATTGTAATTCTTTTACACGACCATATACAAAGACAAGT
ACCAAAATATACATCTGTTTTTTGTCAACACAAATGTATACGCATAAAAGTGATAGATAC
CGAAAGTTAGATATTCCATATGGTGGTGTAACTGAAATTTGGATTGAAGTTGAATATTAT
TTGATTTATTTACGGATCTTTCGG
   
Sequence Name 30-K101331-0022-655-G04C-JV41
GenBank Accession LN499646 [GenBank]
Sequencing primer gene specific primer, JV41: 5'-CCGAAAGATCCGTAAATAAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101331-0022-655-G04C-JV41
ATTTAGTTACACCACCATATGGAATATCTAACTTTCGGTATCTATCACTTTTATGCGTAT
ACATTTGTGTTGACAAAAAACAGATGTATATTTTGGTACTTGTCTTTGTATATGGTCGTG
TAAAAGAATTACAATCATGCACAAGTTGTGGTAGAATCGGCACGAGGTTTTTTAATGTTA
GTGGTGAGGGAATTTCGAAACGTGTTTAAAACTCAAACAAATGGCTGAAAAGAGTTGAAA
AGCTTTGTTATACCCACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11