Sequences from confirmation amplicon


Confirmation sequences for line 657E09 and hit At3g11250 are shown.
Line ID 657E09
Locus At3g11250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3522800
Primers used for PCR CQ57 - 8474, show primer details
   
Sequence Name 38-K107033-0022-657-E09C-8409
GenBank Accession LN502997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K107033-0022-657-E09C-8409
GAGCCATTTACAATTGAATATATCCTGCCGCAATCTACATGGATCACCCTCATAGTCTTC
TTCGTCTGATTCTTCTTTCTTCTCCTCTACCTTAGCTGATTCTCCAGCTTGAGCACTACC
ACCACCTGCATCCGCAGACACTGCCGCTGCAGCAACAACAAACTTGCTGGGATCCTGCAT
TTCAAATTTACACCAAAGAGTTAACATTAACACGAACAATACTACAAGTTTCAAAAACCA
CATAAGAAGAAGATGTACAATTTAATGACTAACCTTTAAGAATTCCTTGACCTTCTCTGC
TTGAGGGAAAGTGTAGTCAGTAGCAACAGCAATAGCTAAAGCATTCTTGTAGGCATTGAT
GAACATATGCGGTGCAGCCGCAAGGGTAGGGTAAGAAACAGCTAGAGCCAAGG
   
Sequence Name 46-K107034-0022-657-E09C-CQ57
GenBank Accession LN505875 [GenBank]
Sequencing primer gene specific primer, CQ57: 5'-TCCTTGGCTCTAGCTGTTTCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K107034-0022-657-E09C-CQ57
TGCCTACAGAATGCTTTAGCTATTGCTGTTGCTACTGACTACACTTTCCCTCAAGCAGAG
AAGGTCAAGGAATTCTTAAAGGTTAGTCATTAAATTGTACATCTTCTTCTTATGTGGTTT
TTGAAACTTGTAGTATTGTTCGTGTTAATGTTAACTCTTTGGTGTAAATTTGAAATGCAG
GATCCCAGCAAGTTTGTTGTTGCTGCAGCGGCAGTGTCTGCGGATGCAGGTGGTGGTAGT
GCTCAAGCTGGAGAATCAGCTAAGGTAGAGGAGAAGAAAGAAGAATCAGACGAAGAAGAC
TATGAGGGTGATCCATGTAGATTGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA

Last Updated on 06.12.2018 16:11