Sequences from confirmation amplicon


Confirmation sequences for line 657F04 and hit At2g17430 are shown.
Line ID 657F04
Locus At2g17430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7569273
Primers used for PCR RP12 - 8474, show primer details
   
Sequence Name 20-K027365-022-657-F04C-8409
GenBank Accession LN495067 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K027365-022-657-F04C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGATCCATGG
CTTCAATTGGTTACAATTGAGATAGAAGGAAATGGAGAGATGGAAGATTTAGAAAAAAAA
ACTCACAGAGATGAATCCATGGCGCAGAGTCAAGTAGTCGGTTCTTTCAACAGATACAAA
GAACTGCCTAAAGAAGCATCCCTGTAACCAAAGAGCATATAATAAATCGAATGAAGATCA
TTTTGATCATTTGAAAGAGATGAAGAATGAGAGAAATGGTTTCTTTACGACGTAAAAGAA
GAAAGGAGTTGTTGTCCAGAAACTTGTATGTTCTCTCACAAAAGAAGTCTCATGAGTGAG
CCTAAGTCTTGAATGATCTGCACCACAGAACAGAAGTAAGTCTGTAAAAGAACAACACTT
CAGTTCAGGGGTACAAAACAGAATCTGATGAACAAAGTACCAATAGAAAACTCATAGTCA
TTAGATAATGTCTC
   
Sequence Name 24-K027365-022-657-F04C-RP12
GenBank Accession LN496856 [GenBank]
Sequencing primer gene specific primer, RP12: 5'-AAAAAGTGGGAGCAAGAGACATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027365-022-657-F04C-RP12
TAATGACTATGACTTTTCTATTGGTACTTTGTTCATCAGATTCTGTTTTGTACCCCTGAA
CTGAAGTGTTGTTCTTTTACAGACTTACTTCTGTTCTGTGGTGCAGATCATTCAAGACTT
AGGCTCACTCATGAGACTTCTTTTGTGAGAGAACATACAAGTTTCTGGACAACAACTCCT
TTCTTCTTTTACGTCGTAAAGAAACCATTTCTCTCATTCTTCATCTCTTTCAAATGATCA
AAATGATCTTCATTCGATTTATTATATGCTCTTTGGTTACAGGGATGCTTCTTTAGGCAG
TTCTTTGTATCTGTTGAAAGAACCGACTACTTGACTCTGCGCCATGGATTCATCTCTGTG
AGTTTTTTTTTCTAAATCTTCCATCTCTCCATTTCCTTCTATCTCAATTGTAACCAATTG
AAGCCATGGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11