Sequences from confirmation amplicon


Confirmation sequences for line 658A06 and hit At4g02270 are shown.
Line ID 658A06
Locus At4g02270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:992189
Primers used for PCR ND08 - 8474, show primer details
   
Sequence Name 02-K032412-022-658-A06C-ND08
GenBank Accession LN486920 [GenBank]
Sequencing primer gene specific primer, ND08: 5'-AGCTTCTCTCATCGTCTTCTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K032412-022-658-A06C-ND08
GGCATGAAGCTGACCCGGAGACCGGAAAATTGATTCCCATCGCCGTTGAAGGGATCATCA
AGTGCAAGTCCGGTGGCAAAACTTACCCAATTCAAGGTAACATAACTTCTTTAATTATAT
ATACCAAGACGAATTGGTCTAATGGTAGGAGAAAATTAGAGCATAGTAAGGTCTAACCAA
ACGAATATCGTATTGTTGTTTGTTTGCGACAGGTGCAACGGCAAGAATTGCGTGTGTGAA
GGTCGATGCATATGGGAATGAGTTAGTTCCAATATCGATATTGAGCAGCAAAACTGATGC
TAAAGGTTACTTCATCGCCACGATATTCCCTTCGCAGCTTCGTGCAGGAAGGACAGTGAC
CAAATGCAAAACTTACCTTTACAAATCTCCACTCGCTGATTGCGATTTTCCGACCGATGT
GAACAAAGGTGTTAGAGGACAGCCATTGAGCACGTACCGTATTCTTCAAGACAAGAGCTT
CAAGCTTTACTGGGCTGGTCCTTTCTTCTACACGTCTGAACCTACTTACTACTAATTATG
ATCTTCTGATGAACATGAAATTTAACATGTTCTTGGAGAATCATTTTTCGTTTGTTTGAT
TATTCGTCGTATAACGTTGTCTGCAAAATGATCTAAGAGTTTGTTTTCTTTATCTTATAT
TTCATGTTGCATATATGACTTTGTCATGTATCTTATGTTTTAATATTTTCTTGGTAAATG
TATGGGTTTCTTGCTTATTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAACAAATT
TATCTAT
   
Sequence Name 02-K032412-022-658-A06C-8409
GenBank Accession LN486919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K032412-022-658-A06C-8409
GCCATTTACATTGAATATATCCTGACAAATAAGCAAGAAACCCATACATTTACCAAGAAA
ATATTAAAACATAAGATACATGACAAAGTCATATATGCAACATGAAATATAAGATAAAGA
AAACAAACTCTTAGATCATTTTGCAGACAACGTTATACGACGAATAATCAAACAAACGAA
AAATGATTCTCCAAGAACATGTTAAATTTCATGTTCATCAGAAGATCATAATTAGTAGTA
AGTAGGTTCAGACGTGTAGAAGAAAGGACCAGCCCAGTAAAGCTTGAAGCTCTTGTCTTG
AAGAATACGGTACGTGCTCAATGGCTGTCCTCTAACACCTTTGTTCACATCGGTCGGAAA
ATCGCAATCAGCGAGTGGAGATTTGTAAAGGTAAGTTTTGCATTTGGTCACTGTCCTTCC
TGCACGAAGCTGCGAAGGGAATATCGTGGCGATGAAGTAACCTTTAGCATCAGTTTTGCT
GCTCAATATCGATATTGGAACTAACTCATTCCCATATGCATCGACCTTCACACACGCAAT
TCTTGCCGTTGCACCTGTCGCAAACAAACAACAATACGATATTCGTTTGGTTAGACCTTA
CTATGCTCTAATTTTCTCCTACCATTAGACCAATTCGTCTTGGTATATATAATTAAAGAA
GTTATGTTACCTTGAATTGGGTAAGTTTTGCCACCGGACTTGCACTTGATGATCCCTTCA
ACGGCGATGGGAATCAATTTTCCGGTCTCCGGGTCAGCTTCATTGCCGTAATAAGCAACT
GAAGATATGATTAGAGATGAGA

Last Updated on 06.12.2018 16:11