Sequences from confirmation amplicon


Confirmation sequences for line 658A12 and hit At5g57150 are shown.
Line ID 658A12
Locus At5g57150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23154529
Primers used for PCR 27uv - 8474, show primer details
   
Sequence Name 64-K118051-0022-658-A12C-27uv
GenBank Accession LN511291 [GenBank]
Sequencing primer gene specific primer, 27uv: 5'-ACATTTATTGGGTTGTGTGGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118051-0022-658-A12C-27uv
GTTTATAAAAAGAAAATAATATTATCTCTGTGGTAAGTAGAATCTGATTGCTTTGATATT
AAAATATTGTTCAACTGCTCGAATTAAATACAATAGGATAAGAAATTATAGAAATGATAG
AATATAGCTAATTCTGCCAAATACATCATATATTGTCTGTCTGGAGATAATAAAAGATAG
CTGATTCTATCAAATACCTCACATAGAAAAACAAACATAAAGAGTCAATAATTATTGGAT
CTTTTAGAGATAAGATTATGATTGCATTTGCTAATTTTTTTTTTTTTGGGCCCCAAATGG
CATTGGCAATTAAAAAAAAAGAACGGGTCCTGGTTCCCTTTTTTTTAAGGGGGGGGATTT
GGGAACCATTCTTTTTTTGGGCCCGGGGTTTTTTCAATTTGAAAAGGGTTCCTGGCCGGG
AAATTCTCCGGGGATCCCCATGGATTTTGAGGGCAAATTGGGAAAAAAAAAAAGAGTATT
TCCCATTTTTTTTTCATTTCAAAATTGTAATTTCC
   
Sequence Name 28-K118050-0022-658-A12C-8409
GenBank Accession LN499033 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118050-0022-658-A12C-8409
TTGAATTAGCCATGAGCCAAAGAAGAGACTGGTCCCATACTCCCCCCATTAAAGATAAAG
GAATCAAGTACATGTACTATTATTTAATTAGCAATTGCAATCTGCTGCCAAAAAAAAAAA
AATTTACAAAGGGCATCCAAATTTTTTTCCCAAAAAAACCCAAAATTTTTGGCCCCTTTA
GTTTGTTTTTCCTTGGGGGGGTTTTGAAAAAAACCCCTTTTTTTTTTTTTCCCCCAAACA
AAAAATTTTTGATTTTTTGGGAAAATTTACCTATTTCCTACCTTTTCTAAATTTCTTTAC
CCTTTGGTTTTAATTCCGGCCGTTTAAACATTTTTTTAATTTCAAACCATTCAATTTCTC
TTTCCCCCAAAAAAAATTTTTTTTTTTTTTAAAAAACCCAATGGGCCCAATTTCCATTAT
TTGGGGACCCAAAATTAACCTTAAAACCTAAAACCCCCCCA

Last Updated on 06.12.2018 16:11