Sequences from confirmation amplicon


Confirmation sequences for line 658B07 and hit At1g15210 are shown.
Line ID 658B07
Locus At1g15210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5233179
Primers used for PCR NA47 - 8474, show primer details
   
Sequence Name 53-K101565-0022-658-B07C-8409
GenBank Accession LN508098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101565-0022-658-B07C-8409
ATTTACATTGAATAATAAAATACTTAATTGTAACAGTAACCAGAGATTCTAGCAAAAGTC
TCTTGTTTCTTTGGGAATCCAGATACTCTTACGTCTCCTTCTATGTATCCACCTGTTTTC
CTTCCGGCCAAAACGTCCATCAAAGTTGTCTTACCCGCACCGCTCACCCCCATCAACGCA
GTTAAAACTCCTGGCCTAAATGCGCTTGTCACTCCTTTTAGAAGTTGCAGCCTCGTTTCT
TGAACTCCTTGTTCCCTCATTTCCTGCACCATCCAATTCTATATGAGTTTCTTTTTTTGT
TTCTATTAATAATAACAATAAGTTTAACTAGTTTTCAAAGTATTACCGCAGGCATGTCAA
CAAAGTATTTAACATCGTCAAATGACATAGCTNGAGGAGTGAAAGGAAGAACCATTCCTT
TCTTGGCGCTCACACTCTCCATCTCAGTCTCCTCTGTTTTCCATAAAAGAGTAAATTCTT
AATATTATTGTTTTTTGGTTTATATAATGAATTCTTAAGATGTAAACAATGGAATATACT
TACTATTGGATCCAGCTTTGCCTTTAGCCTCTTCGTCTTCTTCTTTTGGAAGTAAGCTGG
GTGCC
   
Sequence Name 53-K101578-0022-658-B07C-NA47
GenBank Accession LN508099 [GenBank]
Sequencing primer gene specific primer, NA47: 5'-AAGGCACAAGCTATACTTCCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101578-0022-658-B07C-NA47
TGGATCCATAGTAAGTATATTCCATTGTTTACATCTTAAGAATTCATTATATAAACCAAA
AAACAATAATATTAAGAATTTACTCTTTTATGGAAAACAGAGGAGACTGAGATGGAGAGT
GTGAGCGCCAAGAAAGGAATGGTTCTTCCTTTCACTCCTCTAGCTATGTCATTTGACGAT
GTTAAATACTTTGTTGACATGCCTGCGGTAATACTTTGAAAACTAGTTAAACTTATTGTT
ATTATTAATAGAAACAAAAAAAGAAACTCATATAGAATTGGATGGTGCAGGAAATGAGGG
AACAAGGAGTTCAAGAAACGAGGCTGCAACTTCTAAAAGGAGTGACAAGCGCATTTAGGC
CAGGAGTTTTAACTGCGTTGATGGGGGTGAGCGGTGCGGGTAAGACAACTTTGATGGACG
TTTTGGCCGGAAGGAAAACAGGTGGATACATAGAAGGAGACGTAAGAGTATCTGGATTCC
CAAAGAAACAAGAGACTTTTGCTAGAATCTCTGGTTACTGTTACAATTAAGTATTTTATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCC

Last Updated on 06.12.2018 16:11