Sequences from confirmation amplicon


Confirmation sequences for line 659D12 and hit At3g18160 are shown.
Line ID 659D12
Locus At3g18160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6221749
Primers used for PCR 27st - 8474, show primer details
   
Sequence Name 35-K116478-0022-659-D12C-27st
GenBank Accession LN501967 [GenBank]
Sequencing primer gene specific primer, 27st: 5'-GGAAATGACGTTGGTAAAGATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116478-0022-659-D12C-27st
CTAGACCATGAATACCTAATTTCGATAATTTTGAAAGGAAAAGATGCAAAATATCAACTT
CTACCCCTTGAAATCTCAATTTGAAATTTTGATATCTCAAGATACAATGCAACAAGTAGA
ATGAAAGTACACAAAATTTTATCAGAATGACCAATTTGCTAGAAGTAGCTTAACAATGAT
TCTTAAGACAATAAAAAGAGAAAAGTAAAATGTCTTACAAGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 10-K116477-0022-659-D12C-8409
GenBank Accession LN490819 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116477-0022-659-D12C-8409
ATTACTTGTAGACATTTTACTTTTCTCTTTTTATTGTCTTAAGAATCATTGTTAAGCTAC
TTCTAGCAAATTGGTCATTCTGATAAAATTTTGTGTACTTTCATTCTACTTGTTGCATTG
TATCTTGAGATATCAAAATTTCAAATTGAGATTTCAAGGGGTAGAAGTTGATATTTTGCA
TCTTTTCCTTTCAAAATTATCGAAATTAGTGTATTCATTGGTCTTAGATATAACTCTTGG
CTGGATTACCACAATCTTTACCACGNTCATTTCC

Last Updated on 06.12.2018 16:11