Sequences from confirmation amplicon


Confirmation sequences for line 659G11 and hit At1g23870 are shown.
Line ID 659G11
Locus At1g23870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8434092
Primers used for PCR FQ79 - 8474, show primer details
   
Sequence Name 03-K033883-022-659-G11C-FQ79
GenBank Accession LN487379 [GenBank]
Sequencing primer gene specific primer, FQ79: 5'-CAAACTTATAGCCATGGAACGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K033883-022-659-G11C-FQ78
TATGCGAGGAAACTTGTCCTGATTCAGATAGTGAACCCAGCACGGGCCACGGGTAAGGAT
GTGGAAGAAGCAAAGAAGGAGACATATTCAACTGCAAAAAGGATCAATGAGCGCTATGGT
TCTGCTGGTTATCAGCCAGTGATCTTGATTGATCGTCTTGTTCCTCGTTACGAGAAGACT
GCTTATTATGCTATGGCAGACTGCTGCCTGGTGAATGCAGTAAGAGACGGCATGAACTTA
GTTCCATATAAATATATCATTTGCAGGCAAGGGACCCCAGGAATGGATAAGGCCATGGGG
ATTGGCGGAATGCTTATTCAATTGATGAGTATGACCCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 43-K033883-022-659-G11C-8409
GenBank Accession LN504750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K033883-022-659-G11C-8409
TGAAGCCATTTACAATTGAATATATCCTGGGGGTCATACTCATCAATTGAATAAGCATTC
CGCCAATCCCCATGGCCTTATCCATTCCTGGGGTCCCTTGCCTGCAAATGATATATTTAT
ATGGAACTAAGTTCATGCCGTCTCTTACTGCATTCACCAGGCAGCAGTCTGCCATAGCAT
AATAAGCAGTCTTCTCGTAACGAGGAACAAGACGATCAATCAAGATCACTGGCTGATAAC
CAGCAGAACCATAGCGCTCATTGATCCTTTTTGCAGTTGAATATGTCTCCTTCTTTGCTT
CTTCCACATCCTTACCCGTGGCCCGTGCTGGGTTCACTATCTGAATCAGGACAAGTTTTC
CTCGCATATGCCAATATGTCTCAAAGAGACGTTCCATGGCTATAAGTTT

Last Updated on 06.12.2018 16:11