Sequences from confirmation amplicon


Confirmation sequences for line 661G12 and hit At4g13095 are shown.
Line ID 661G12
Locus At4g13095 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7635515
Primers used for PCR 23qt - 8474, show primer details
   
Sequence Name 13-K115950-0022-661-G12C-23qt
GenBank Accession LN492192 [GenBank]
Sequencing primer gene specific primer, 23qt: 5'-AAAAACGTTCTTCTCTATCCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115950-0022-661-G12C-23qt
TTATCCTCAACTTTTTGCAGCATGGCCAAACAATTGTGTTCTTATATGTTCATCTCCATG
TTTATTCTCTCAGGTACAAATTCGTATACTAAACACGTTATAAAACTCAAATATAAACTC
TTGAGATCGATGAGCAGAGACGGTCATTCTTCAATCTTTTTAGAAAACTAGTGAAACATA
TAATTATCTAGAGTTACGAAAATGAGTAGATGATTAGTTATGGGATGATCAATTGGCATC
TAAATATTGTTTTTAAAAACTATGCTTTTTTTGTTTATTTAATATACTCAATTTTATTTG
TAAGCGTTTTAAAATATTATTTGTTGTTAGCTTTTTTGGCTTTGCCAAGTGCAGAGGGAG
GAGCGACTATAAAAAAATGTGTTGTGGACGTGAAACTTAGCAAGCCATGCACTTTTCAAG
AATGCCAACCACTTTGTCTTCAAAAGTATAACGGGAATGGCCTTTGTCCCGGAGATGACA
ACAATATTTGCGCTTGTGTTTACAATTGCTAAGCTAATTAGAGATATCTCTCGTATGTTA
TAAATGCAGATATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 03-K115949-0022-661-G12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115949-0022-661-G12C-8409
CCCGTTATACNTNNTTTGAAGAACAAAGTGNNGTTGGCATNNNTCTTGAAANNAGTGCAT
GGCNTNNTGCTAAGTTTCACNGTCNNCACAACACATTTNNTTTTATAGTCNNGCTCCTCN
NCCTCTNNNCACTTGGCANNAAGCCAAAAAANNGCTAACAACNNAAATAATAANTTTTAA
AANNNGCTTACAAANNTAAAATTGAAAGTATATTANNAATAAACNNAAAAAAAGCANNTA
GTTTTTANNAAAACAATNNATTTAGATGNCCNNAATTGATCANNTCCCATAANNCTAATC
ATCTNNACTCATTTTNNCGTAACTCTAGAATAAATTNAATATGTTTNNCACTAGTTNNTT
CTAAAAAGAAATTGAAGAAATGAACCGNTNNCTCTGCTNNCATCGATNNCTCAAGAAAGT
TTAT

Last Updated on 06.12.2018 16:11