Sequences from confirmation amplicon


Confirmation sequences for line 664F02 and hit At3g28290 are shown.
Line ID 664F02
Locus At3g28290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10548153
Primers used for PCR 33vs - 8474, show primer details
   
Sequence Name 71-K117997-0022-664-F02C-33vs
GenBank Accession LN512627 [GenBank]
Sequencing primer gene specific primer, 33vs: 5'-ACATACTAGAACCATGGATCCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K117997-0022-664-F02C-33vs
TTCGAAATTTACTCCATTTCTTTCGACTACTTATTTCCATCTCAATATTTTTAAGTTCCT
CATCAAGCTTTTCCTTAGTCTCCCTCACTCTTTCCAGAAGGGATTCTTGCTCCTTGTGCA
TCAACTTGAACAGAGTCGTGAACTTATCGCCATCAAAAGGATTTCCCATGGCTTCAAACT
TCTTCAGCTCCCTCAAGGTATTTTCATACCTCTTCGTTTTTTTGCCAACATCATTTTCTT
TGTCTTTTTCAAACTGTGCCACGGCCGCTACAATGTAACGTTCGCCCCTTTTTGCTTCAT
CGACGTATTCTGTTACATTCTCGGCAATGTCCAAAGTCTTCTTGGCACTTTCAAAATAGG
TTTCGACCAAAGATCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 31-K117996-0022-664-F02C-8409
GenBank Accession LN500332 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117996-0022-664-F02C-8409
CATTTACATTGAGATCTTTGGTCGAAACCTATTTTGAAAGTGCCAAGAAGACTTTGGACA
TTGCCGAGAATGTAACAGAATACGTCGATGAAGCAAAAAGGGGCGAACGTTACATTGTAG
CGGCCGTGGCACAGTTTGAAAAAGACAAAGAAAATGATGTTGGCAAAAAAACGAAGAGGT
ATGAAAATACCTTGAGGGAGCTGAAGAAGTTTGAAGCCATGGGAAATCCTTTTGATGGCG
ATAAGTTCACGACTCTGTTCAAGTTGATGCACAAGGAGCAAGAATCCCTTCTGGAAAGAG
TGAGGGAGACTAAGGAAAAGCTTGATGAGGAACTTAAAAATATTGAGATGGAAATAAGTA
GTCGAAAGAAATGGAGTATAATTTCGAATGTGCTTTTCATCGGTGCGTTTGTTGCTGTTG
CCGTCGGATCCATGGTCNTAGTATGTAAAAA

Last Updated on 06.12.2018 16:11