Sequences from confirmation amplicon


Confirmation sequences for line 665D07 and hit At3g23970 are shown.
Line ID 665D07
Locus At3g23970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8660470
Primers used for PCR 43qn - 8474, show primer details
   
Sequence Name 68-K115691-0022-665-D07C-43qn
GenBank Accession LN512073 [GenBank]
Sequencing primer gene specific primer, 43qn: 5'-CTATGGAGGTCATGATGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115691-0022-665-D07C-43qn
GATGATGATCANTTACGGCGTTTTAGGAGAACATTGTCGACCTCGGATGGGTCTATTGTC
TACTTCAACGAGTTTGGTGAGAACGGAAACCGAAGATTACGTGTTTGGAGGCTGGTCAAG
TACACTGGTGGTCCTGAGGCGTGGCAGCTCTTTTGGGAAGTCAGCTTGGCGTCTGTGACC
AAGCTAGGTATCGATTACTTCCCGGTGGTGATGCATCCTTTGAAGTCTGAGATCATCTAC
TTGTGGAGCAGAAACAAGAAAGGTATGGTTTTGTTTAACTTGAGGACTAATGTGTTTAGT
CTTCACAAGGAATCCGAGGACGAGACCAAGTGTATGGATGGTTGCACCTTGAGCTTTAAC
AGGTGCAGCGAGTATATGGAGACTATCCATAACTATGGTGGTCCTAACTATCTCCTCGCT
TCACAGTATGTTCTTCCTCGTTGGCTGCACCGTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 20-K115690-0022-665-D07C-8409
GenBank Accession LN495351 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115690-0022-665-D07C-8409
ATTTACAATTGACGGTGCAGCCAACGAGGAAGAACATACTGTGAAGCGAGGAGATAGTTA
GGACCACCATAGTTATGGATAGTCTCCATATACTCGCTGCACCTGTTAAAGCTCAAGGTG
CAACCATCCATACACTTGGTCTCGTCCTCGGATTCCTTGTGAAGACTAAACACATTAGTC
CTCAAGTTAAACAAAACCATACCTTTCTTGTTTCTGCTCCACAAGTAGATGATCTCAGAC
TTCAAAGGATGCATCACCACCGGGAAGTAATCGATACCTAGCTTGGTCACAGACGCCAAG
CTGACTTCCCAAAAGAGCTGCCACGCCTCAGGACCACCAGTGTACTTGACCAGCCTCCAA
ACACGTAATCTTCGGTTTCCGTTCTCACCAAACTCGTTGAAGTAGACAATAGACCCATCC
GAGGTCGACAATGTTCTCCTAAAACGCCGTAACTGATCATCATCTTTTTCACAATCAGGG
AAAGATATGATATGACATGCATAATCATCAGACCNTTCCATA

Last Updated on 06.12.2018 16:11