Sequences from confirmation amplicon


Confirmation sequences for line 668B10 and hit At1g80240 are shown.
Line ID 668B10
Locus At1g80240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30171550
Primers used for PCR CQ68 - 8474, show primer details
   
Sequence Name 23-K107131-0022-668-B10C-CQ68
GenBank Accession LN496579 [GenBank]
Sequencing primer gene specific primer, CQ68: 5'-TGATTAAGTGGTGACCTCTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K107131-0022-668-B10C-CQ68
GGATTCGAGAAGACCTTACGTGTTTCCACGGCAAAATGGGGAGTACTGATCCCACCGTTC
ATCGAGGACGATAACAGCCCATTGCCCGGTTGGATGATCGAGTCTCTCAAAGCCGTTAAG
TACGTAGACAAAGCACACTTCGCTGTCCCCGAAGGACACCGAGCCATCGAGCTTGTTGGA
GGCAAAGAGAGCGCCATTTCTCAGATAGTGAGGACGTCACTGAACAAATTCTACGCCCTC
ACGTTCAACGTGGGAGACGCCAGAGACGGTTGTGAGGGACCAATGATTGTGGAAGCGTTC
GCTGGACAGGGCAAGGTCATGGTGGACTACGCTTCTAAAGGAAAAGGCGGGTTTAGACGT
GGGAGGCTTGTGTTCAAGGCAGTGTCGGCCAGGACACGTGTCACTTTCTTGAGCACGTTT
TACCACATGAAAAGCGATCACTCTGGCTCGCTATGTGGTCCGGTGATCGATGATGTTAGG
GAAACTCCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCC
   
Sequence Name 07-K107130-0022-668-B10C-8409
GenBank Accession LN489296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107130-0022-668-B10C-8409
TTACAATTGGGAGTTTCCCTAACATCATCGATCACCGGACCACATAGCGAGCCAGAGTGA
TCGCTTTTCATGTGGTAAAACGTGCTCAAGAAAGTGACACGTGTCCTGGCCGACACTGCC
TTGAACACAAGCCTCCCACGTCTAAACCCGCCTTTTCCTTTAGAAGCGTAGTCCACCATG
ACCTTGCCCTGTCCAGCGAACGCTTCCACAATCATTGGTCCCTCACAACCGTCTCTGGCG
TCTCCCACGTTGAACGTGAGGGCGTAGAATTTGTTCAGTGACGTCCTCACTATCTGAGAA
ATGGCGCTCTCTTTGCCTCCAACAAGCTCGATGGCTCGGTGTCCTTCGGGGACAGCGAAG
TGTGCTTTGTCTACGTACTTAACGGCTTTGAGAGACTCGATCATCCAACCGGGCAATGGG
CTGTTATCGTCCTCGATGAACGGTGGGATCAGTACTCCCCATTTTGCCGTGGGAAACACG
TAAGGTCCTTCTTCGAAATTCCCATTCTTTATCAGATTATCTGCAAACAGAGGTCACANC
TTTAATC

Last Updated on 06.12.2018 16:11