Sequences from confirmation amplicon


Confirmation sequences for line 668G11 and hit At3g23760 are shown.
Line ID 668G11
Locus At3g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8563342
Primers used for PCR 57gp - 8474, show primer details
   
Sequence Name 50-K112250-0022-668-G11C-57gp
GenBank Accession LN507215 [GenBank]
Sequencing primer gene specific primer, 57gp: 5'-GAAATACAGCGAAAAGAAACTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112250-0022-668-G11C-57gp
TTCATGGAGAGTATGGCTTAGATCTCCGTGTTTGTTGTAAGCAAATGGACGTTTTCGTGT
CAATGTGGGAGTGTAGCATGTAATGCTTCTGTTTCTTCTTTGATTGATGAATCTATGATC
ATTTGAATATCAACAATTAAGTGATGGTCATGATAAGCTGCAATGAGCCACAATAGGAAT
CTAAGAAATAAGAATAGTGAGTAGGACTTGGTAATATTTTGGACTGCAGTTATATAGAGG
TTCCAAGTCATTCATCCTTGAGTTTTTGATGGGAAATTTTTGATTGTGCTTATGATAGAA
ACATAAGGTTCCACGGTATTGTTTTCAGTGATACACTCACTGACTCAAGTATATGCCTAG
GTAAAGTTGTACGGTAGATTTTGTAGTTCTTTATTGGTTTCAGAAACAGAAATGTTTTAG
TATGTTGCTGAGACTAGTCTTGGTTGACAATATATTGTTGAATATATTGGCCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
C
   
Sequence Name 40-K112249-0022-668-G11C-8409
GenBank Accession LN503752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112249-0022-668-G11C-8409
GCATTTACATTGAATATATCCTGGCCATATATTCAACAATATATTGTCAACCAAGACTAG
TCTCAGCAACATACTAAAACATTTCTGTTTCTGAAACCAATAAAGAACTACAAAATCTAC
CGTACAACTTTACCTAGGCATATACTTGAGTCAGTGAGTGTATCACTGAAAACAATACCG
TGGAACCTTATGTTTCTATCATAAGCACAATCAAAAATTTCCCATCAAAAACTCAAGGAT
GAATGACTTGGAACCTCTATATAACTGCAGTCCAAAATATTACCAAGTCCTACTCACTAT
TCTTATTTCTTAGATTCCTATTGTGGCTCATTGCAGCTTATCATGACCATCACTTAATTG
TTGATATTCAAATGATCATAGATTCATCAATCAAAGAAGAAACAGAAGCATTACATGCTA
CACTCCCACATTGACACGAAAACGTCCATTTGCTTACAACAAACACGGAGATCTAAGCCA
TACTCTCCATTGAATGAAATAAAGCACCACAAAAACATAGGATAGAGACTCTCCAAGTTT
CTTTCCTGNNAAATTTCATTCAATGGAGAGTATGGCTTAGATCT

Last Updated on 06.12.2018 16:11