Sequences from confirmation amplicon


Confirmation sequences for line 669A05 and hit At1g56170 are shown.
Line ID 669A05
Locus At1g56170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21025622
Primers used for PCR CL13 - 8474, show primer details
   
Sequence Name 19-K028745-022-669-A05C-8409
GenBank Accession LN494624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028745-022-669-A05C-8409
GCATTTAACTGGCCCACTCTGATAAGCATGAAGAGGTACATAATCCATCACTCCCTGTTG
CTGTTGCTGTTTACCCTCTTCTGACTGCTCC
   
Sequence Name 19-K028745-022-669-A05C-CL13
GenBank Accession LN494625 [GenBank]
Sequencing primer gene specific primer, CL13: 5'-AGAGATAGCTTAGCAATGGAGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028745-022-669-A05C-CL13
GGATTATGTACCTCCTCATGCTTATCAGAGTGGGCCAGTAAATGCAGCTTCCCATATGGC
ATTCCAACAAGCTCACCACTTCCACCACCACCATCAGCAGCAACAACAGCAGCAGCTTCA
GATGTTCTGGGCTAACCAAATGCAAGAGATCGAGCATACCACTGATTTCAAGAACCACAC
CCTTCCCCTAGCCCGCATCAAGAAGATCATGAAAGCTGATGAAGATGTGAGGATGATCTC
TGCGGAGGCTCCTGTGATTTTTGCCAAGGCCTGTGAGATGTTCATTTTGGAGCTCACTCT
ACGTGCTTGGATCCACACCGAGGAGAACAAGAGGAGGACCTTGCAGAAGAACGACATCGC
CGCTGCCATTTCCAGGACCGACGTGTTTGATTTCCTTGTGGACATAATCCCGAGGGACGA
GCTGAAAGAAGAAGGTTTAGGCGTGACCAAAGGGACCAGCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11