Sequences from confirmation amplicon


Confirmation sequences for line 669B05 and hit At5g08650 are shown.
Line ID 669B05
Locus At5g08650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2809801
Primers used for PCR 03ke - 8474, show primer details
   
Sequence Name 51-K113379-0022-669-B05C-03ke
GenBank Accession LN507564 [GenBank]
Sequencing primer gene specific primer, 03ke: 5'-GGATAAAAACCAAGTTGAGGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113379-0022-669-B05C-03ke
TACGTATCGCTCCTAGACCAAATGTGAATATTTTTAACAAGATACATACAATAGAGGACT
GAAGACCAGTTCTTTGCAAAACACAATTTTACTTTCCAATTCGAAGCCAACTGGGCATAA
ACATACATTGGCTAAGTAAGCATACGAAGACCATAGTTGTTTCGTCCGTGCAAATTATTC
TGGGTACAGATGAAAGAAAAATGTTGTTTCTGTCATTTAATTCTACTATACCTTCAATGC
AGCATCATTGAGTTGCAGTTTTTCCAATGCATCGCGAAGATCGGGAAACCTGCCATCATA
GACCAGGTGTGAAAAAAAAAAATACAAAATGAAAAAGCAAAAAGTCAACAATGAAAAACA
AAAACTCAGTACTGGTCAGCGTCAACTGGAAAAAGACCACAGAACACCATAGGGGTAGCT
TCCTCGTAACCAGGTAAAGAGCTTTCTGCCTTTCTGCTATAAGGTGTTATTGTATCTCCT
ACCCTGGCATCTGCAACAGATCTTACAGAAGCCGCAATATATCCTACCTGTATTTAAGCC
AATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
T
   
Sequence Name 13-K113377-0022-669-B05C-8409
GenBank Accession LN492119 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113377-0022-669-B05C-8409
GCATTTCATTGAATATATTGGCTTAATACAGGTAGGATATATTGCGGCTTCTGTAAGATC
TGTTGCAGATGCCAGGGTAGGAGATACAATAACACATTATAGCAGAAAGGCAGAAAGCTC
TTTACCTGGTTACGAGGAAGCTACCCCTATGGTGTTCTGTGGTCTTTTTCCAGTTGACGC
TGACCAGTACTGAGTTTTTGTTTTTCATTGTTGACTTTTTGCTTTTTCATTTTGTATTTT
TTTTTTTCACACCTGGTCTATGATGGCAGGTTTCCCGATCTTCGCGATGCATTGGAAAAA
CTGCAACTCAATGATGCTGCATTGAAGGTATAGTAAAATTAAATGACAGAAACAACATTT
TTCTTTCATCTGTACCCAAAATAATTTGCACGGACAAAACAACTATGGTCTTCGTATGCT
TACTTAGCCAATGTATGTTTATGCCCAGTTGGCTTCAAATTGGAAAGTAAAATTGTGTTT
TGCAAAGAACTGGTCTTCAGTCCTCTATGGTATGTATCTTTGTTAAAAATATTCACATTT
GGTCTAAGGAGCGTATACTTTAGCATTAACATATCTTTAGGTTTGTCCTCAACTGTTTTT
T

Last Updated on 06.12.2018 16:11