Sequences from confirmation amplicon


Confirmation sequences for line 670B01 and hit At1g72840 are shown.
Line ID 670B01
Locus At1g72840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27412586
Primers used for PCR 15di - 8474, show primer details
   
Sequence Name 64-K109780-0022-670-B01C-15di
GenBank Accession LN511222 [GenBank]
Sequencing primer gene specific primer, 15di: 5'-ATATGTTGACGAGACACTTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K109780-0022-670-B01C-15di
TTTTTAGAATGCTCTGATATATCGGAGATGGCCACCATTAAAGAAACATGCCACATGAAG
GAAAACAGTCTTGTCGTATTGATCTAAACCGTCATAGCTAGCTCTCAAAATCTCTTGGAC
ATTTTTTTGAGGGAATGTTTCAAGTAGAGCTAGCTCATCTTCCCATTCATCTATGGCCAC
AATAGCACTGAGATGGGAGGCAAAAGCTACAAGGGCAGAAGGAAGGCCGTGAGCAAGCCG
AGAAGCTCTGATAAAGAGTTGCTCAAAACCATCAGAAGGAGGTCTTCCCCCAAAAGCTAA
CTTTTTAAAAACCTGAAGCGCATCCTTATCGTCCAAGCACTTAACCTCATATATATTGTT
TACTCCGCAGGAATTGAGCAAACCTTTGTCTCGTGTGGTTATGATGATACGGCTCCCTGG
ACCGAACCAGCTTGGGTCCTTTGCCAGGCCATGTAACTGCTCCACTTTATCGACATTATC
AAGCACGACAAAAACTTCTTGATGCCCCAGTCTCTCCTTTATCTCTTGGGATCCAGCTTC
CATGCTCCATAACTCGACATCTTCATCATAAGAGGATATGGGAAAGCAAATCCTTTT
   
Sequence Name 48-K109779-0022-670-B01C-8409
GenBank Accession LN506527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K109779-0022-670-B01C-8409
GCCATTTGGAAACGTGTCTAAGGCTATGATATTAAGCATCTACAAAAGGACTTGTTTTCC
CATATCCTCTATGATGAAGATGTCGAGTTATGGAGCATGGAAGCTGGATCCCAAGAGATA
AAGGAGAGACTCGGGCATCAAAAAGTTTTTGTCGTGCTTGATAATGTCGATAAAGTGGAG
CAGTTACATGGCCTGGCAAAGGACCCAAGCTGGTTCGGTCCAGGGAGCCGTATCATCATA
ACCACCCGAGACAAAGGTTTGGTCA

Last Updated on 06.12.2018 16:11