Sequences from confirmation amplicon


Confirmation sequences for line 670E09 and hit At5g47670 are shown.
Line ID 670E09
Locus At5g47670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19315494
Primers used for PCR PG15 - 8474, show primer details
   
Sequence Name 61-K101716-0022-670-E09C-8409
GenBank Accession LN510472 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K101716-0022-670-E09C-8409
TTACATTGAATATATCCTCCGCATGATCCGTATCACGTTGGCAATAGGCATGAACCTGTC
TTGCTCCCTCACCGTGCACTCCTCCTCACCACCATTACTGGTTTTGTTAGGCTGGTTGAA
TTCTGGAGGACGCATACTGCCCTCTGCCATCAGAAAATTCGCTGCTAATCCTAACAAAAA
TAAGAAAAATGGAAGTTTTAGTGCATAATGGCTTCGATGACGTCTGCATGCGCGGTTTAT
AAGATTGAAAAGCTATTTCATTAACTGTTCGTCATGAAAAGAGTACAGACATGACAATGG
AGGGATTGCAGAGAATGGTTTCGCATGCAACTGAAATGAAGAGAGCCTGTGAAAAGAGGG
GGTATAGAGAATGGCACTACCTGGTGGAGAAGGAGTGGTGTTGTTCACGGACAGCTTGCG
GTAGCCATGGAAGCCTCCACGTTCCATATCTTTCTTGTTCTAAATACACGACTTTTGTGT
TTGTGAATATCCCTATNAATAATAATAACCAATGCCCGAAATTAAGAAGCATTTGAAGAA
GAGAATGGAATGAGAGAGAAGAAAGAGTAATTGGAATAAAGCTAATAGTGTTGGCAAGTG
TCAGCATTTGTAATTCAAGACCTC
   
Sequence Name 61-K101722-0022-670-E09C-PG15
GenBank Accession LN510473 [GenBank]
Sequencing primer gene specific primer, PG15: 5'-TGAGGTCTGAAATTACAAATGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K101722-0022-670-E09C-PG15
CTCTTTCTTCTNCNCATTCCATTCTCTTCTTCAATGCTTCTTAATTTCGGGCATTGGTTA
TTATTATTTATAGGGATATTCACAAACACAAAAGTCGTGTATTTAGAACAAGAAAGATAT
GGAACGTGGAGGCTTCCATGGCTACCGCAAGCTGTCCGTGAACAACACCACTCCTTCTCC
ACCAGGTAGTGCCATTCTCTATACCCCCTCTTTTCACAGGCTCTCTTCATTTCAGTTGCA
TGCGAAACCATTCTCTGCAATCCCTCCATTGTCATGTCTGTACTCTTTTCATGACGAACA
GTTAATGAAATAGCTTTTCAATCTTATAAACCGCGCATGCAGACGTCATCGAAGCCATTA
TGCACTAAAACTTCCATTTTTCTTATTTTTGTTAGGATTAGCAGCGAATTTTCTGATGGC
AGAGGGCAGTATGCGTCCTCCAGAATTCAACCAGCCTAACAAAACCAGTAATGGTGGTGA
GGAGGAGTGCACGGTGAGGGAGCAAGACAGGTTCATGCCTATGGCCAACGTGATACGGAT
CATGCGGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCANAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11