Sequences from confirmation amplicon


Confirmation sequences for line 675G01 and hit At1g34540 are shown.
Line ID 675G01
Locus At1g34540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12638456
Primers used for PCR 26oc - 8474, show primer details
   
Sequence Name 35-K114782-0022-675-G01C-26oc
GenBank Accession LN501938 [GenBank]
Sequencing primer gene specific primer, 26oc: 5'-CCAGAGGTGGAAGATAAGATTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114782-0022-675-G01C-26oc
GAGCGATCGGGGGAGTTTACGGGTTTGAACATCTTAAGATGATGAATTATCTTCACGCTG
CTATAACTGAATCTTTAAGATTGTATCCACCTGTGCCAGTGGACATAAAGAGTTGTGCTG
AGGACAATGTATTGCCTGATGGGACATTTGTAGGTAAAGGTTGGGCAATAACCTATAACA
TATTTGCAATGGGAAGGATGGAGAGTATTTGGGGGAAAGATTGTGATCGTTTTGATCCTG
AGAGGTGGATTGATGAAACAAATGGTTGTTTTAGAGGTGAGGATCCTTCTAAGTTTCCTG
CGTTTCATGCTGGACCAAGGATGTGTGTAGGGAAGGATATGGCTTATATTCAAATGAAAT
CGATAGTCGCTGCGGTTTTAGAGCGGTTTGTGGTTGAGGTTCCAGGAACATTTGTTGGAG
ATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 35-K114769-0022-675-G01C-8409
GenBank Accession LN501937 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114769-0022-675-G01C-8409
ACCACAAACCGCTTCTAAAACCGCAGCGACTATCGATTTTCATTTGAATATAAGCCATAT
CCTTTCCCTACACACATCCTCGGTTCCAGCATGAAACGCAGGAAACTTAGAAGGATCCTC
ACCTCTAAAACAACCATTTGTTTCATCAATCCACCTCTCAGGATCAAAACGATCACCATT
CTTTCCCCCAAATACTCTCCATCCTTCCCATTGCAAATATGTTATAGGTTATTGCCCAAC
CTTTACCTACAAATGTCCCATCAGGCAATACATTGTCCTCAGCACAACTCTTTATGTCCA
CTGGCACAGGTGGATACAATCTTAAAGATTCAGTTATAGCAGCGTGAAGATAATTCATCA
TCTTAAGATGTTCAAACCCGTAAACTTCCCCGATTCGCTTCCCAGTTCGTGCGCGAATCG
AGTTTAGCTCTTGCAGAATCTTA

Last Updated on 06.12.2018 16:11