Sequences from confirmation amplicon


Confirmation sequences for line 676E04 and hit At1g26270 are shown.
Line ID 676E04
Locus At1g26270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9090494
Primers used for PCR CQ70 - 8474, show primer details
   
Sequence Name 31-K105918-0022-676-E04C-CQ70
GenBank Accession LN500166 [GenBank]
Sequencing primer gene specific primer, CQ70: 5'-ATGCTCCTAATAACCCAAAAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K105918-0022-676-E04C-CQ70
GGTTGAGCGTTCGTACGGGTTGGGGAAACAGGCTACAGAGAAGTTGCGGCTTATCTTCTC
GACAAGGAGCATTTTGCAAATGTTCCTCCCACTGCTCTTGTGAAGATTACTCACTCTATC
TTTAATGTCAACGATGGAGTGAAGGCAAGCAAGCCTATGGAGAAGATGTTGGTGAGCAAG
ATTGCTTCCTTGCAGCAGTTCATACCTCATGATTATGATGCTAGCGAGCACGGAACATCA
AATTTCCCTGTTTCAGCTGTACACCGTATAGGGATTCTTGACATCAGAATCTTAAATACA
GATAGGCACTCAGGGAACCTTTTGGTCAAGAAACTGGATGGTGATGGTATGTTTGGCCAG
GTGGAGCTAGTTCCAATCGATCATGGTCTTTGCTTGCCTGAAACTCTAGAGGACCCTTAC
TTTGAGTGGATTCATTGGCCACAGGCATCGATCCCATTCTCTGAAGATGAGCTGAAGTAC
ATAGCANATCTGGATCCTTTAGGAGATTGTGAGATGCTGCGGAGGGAACTTCCAATGGTA
CGAGAGGCCAGTCTCCGTGTTCTAGTTCTCTGCACAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 31-K105919-0022-676-E04C-8409
GenBank Accession LN500167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K105919-0022-676-E04C-8409
GAGCCATTTACAATTGTGCAGAGAACTAGAACACGGAGACTGGCCTCTCGTACCATTGGA
AGTTCCCTCCGCAGCATCTCACAATCTCCTAAAGGATCCAGATTTGCTATGTACTTCAGC
TCATCTTCAGAGAATGGGATCGATGCCTGTGGCCAATGAATCCACTCAAAGTAAGGGTCC
TCTAGAGTTTCAGGCAAGCAAAGACCATGATCGATTGGAACTAGCTCCACCTGGCCAAAC
ATACCATCACCATCCAGTTTCTTGACCAAAAGGTTCCCTGAGTGCCTATCTGTATTTAAG
ATTCTGATGTCAAGAATCCCTATACGGTGTACAGCTGAAACAGGGAAATTTGATGTTCCG
TGCTCGCTAGCATCATAATCATGAGGTATGAACTGCTGCAAGGAAGCAATCTTGCTCACC
AACATCTTCTCCATAGGCTTGCTTGCCTTCACTCCATCGTTGACATTAAAGATAGAGTGA
GTAATCTTCACAAGAGCAGTGGGAGGAACATTTGCAAAATGCTCCTTGTCGAGAAGATAA
GCCGCAACTTCTCTGTAGCCTGTTTCCCCAACCCGTACTGAACGCTTCAACCCAGGTTGT
CCAAGCGCCTTACCAACAAAACCTTTTGG
Primers used for PCR CQ71 - 8474, show primer details
   
Sequence Name 32-K105918-0022-676-E04C-CQ71
GenBank Accession LN500586 [GenBank]
Sequencing primer gene specific primer, CQ71: 5'-CTTCTTCTTCTTCTTCTTCCCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K105918-0022-676-E04C-CQ71
GAGCATTTCCAACGTGAGTCCGAGTGGTAATGCTAGTTTTTTCGTACAATCAGTTACTTC
CTCACAATCTATATCAAACTGGAATTCAATATCATTTCCCAAGTCTGATCTTGGGGACTC
AGCGTCTTTCTCTCCTATCAAACTCATAGCCTCAAGACAAACCACTTCAATCTCACTCGG
CTCCTCATCTCCAGGACGAACCTCTCTAGTCATCATCTCACCAATTTCAGCCAAACAAAG
GCCATTAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCG
   
Sequence Name 32-K105919-0022-676-E04C-OriL
GenBank Accession LN500587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K105919-0022-676-E04C-OriL
CGGACTGAGCCTTTACATTGAATTATCCTGCTAATGGCCTTTGTTTGGCTGAAATTGGTG
AGATGATGACTAGAGAGGTTCGTCCTGGAGATGAGGAGCCGAGTGAGATTGAAGTGGTTT
GTCTTGAGGCTATGAGTTTGATAGGAGAGAAAGACGCTGAGTCCCCAAGATCAGACTTGG
GAAATGATATTGAATTCCAGTTTGATATAGATTGTGAGGAAGTAACTGATTGTACGAAAA
AACTAGCATTACCACTCGGACTCACGTTTGGAAATGCTCGTGGTCAGTTGTCAAAGGTGG
AAGAAACAACAGAGGATGGGGAAGAAGAAGAAGAAGAAGAA

Last Updated on 06.12.2018 16:11