Sequences from confirmation amplicon


Confirmation sequences for line 676F05 and hit At3g57830 are shown.
Line ID 676F05
Locus At3g57830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21421022
Primers used for PCR DM20 - 8474, show primer details
   
Sequence Name 82-K101748-0022-676-F05C-8409
GenBank Accession LN514170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101748-0022-676-F05C-8409
TTACAATTGAATATATACTCATTTGGATGTTGGACTCTACTTATAGCCTCCACTTCATTT
TCGAAATCCTTCCGCCGCCACGTGGCATCTCCGTCGCTTAGCCTTCTCACAGCAACGACG
GTGGATGACGTAAACGTAGCCGCCACTGTACCTGATCCCATTCCGGCCACTACTCTGTAC
ACAATCCCACTTCTGCTCTTTCCGACGACGTAAGCCGATGCTCTCAGCAAATCCTCGAGC
TCGAGCTCGAATCCTTCGTCCATCACCACGAATTTACCTTCCTTCTCCTCCTCATCCGCC
GCATCATCCAATGGCGCCGCCGTGTTGTTTTTTTCCGGTGTACTCACAGTGGAGCTTAAT
TTTCTTCGAATCAGCCATACGGAGATAGAAACTGCTCCGATTACGATTGAGACTCCGGAG
ATGAGAGAAACCGTTACGGATCCGGTGATCGGTTTATTCTTTCTTCCGTCCTTGTCGATG
AAGCTAGGGTTTGGTTTCTTCGGGAGGATTTGCGAGCCTTCTGGTTTTGGAGCGACGAGC
TTAGGGTTCGTACCTTCATCTTTACACAGCTTCTGTAATGGGAAACCACAGAGCTCAAAG
TTTCCGGCGAACGCTGTTGGTCCTT
   
Sequence Name 82-K101754-0022-676-F05C-DM20
GenBank Accession LN514171 [GenBank]
Sequencing primer gene specific primer, DM20: 5'-CTCTCTTAAACCAAGGACCAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101754-0022-676-F05C-DM20
TCCATTACAGAAGCTGTGTAAAGATGAAGGTACGAACCCTAAGCTCGTCGCTCCAAAACC
AGAAGGCTCGCAAATCCTCCCGAAGAAACCAAACCCTAGCTTCATCGACAAGGACGGAAG
AAAGAATAAACCGATCACCGGATCCGTAACGGTTTCTCTCATCTCCGGAGTCTCAATCGT
AATCGGAGCAGTTTCTATCTCCGTATGGCTGATTCGAAGAAAATTAAGCTCCACTGTGAG
TACACCGGAAAAAAACAACACGGCGGCGCCNTTGGATGATGCGGCGGATGAGGAGGAGAA
GGAAGGTAAATTCGTGGTGATGGACGAAGGATTCGAGCTCGAGCTCGAGGATTTGCTGAG
AGCATCGGCTTACGTCGTCGGAAAGAGCAGAAGTGGGATTGTGTACAGAGTAGTGGCCGG
AATGGGATCAGGTACAGTGGCGGCTACNTTTACGTCATCCACCGTCGTTGCTGTGAGAAG
GCTAAGCGACGGAAATGCCACGTGGCGGCGGAAGGATTTCGAAAATGAAGTGGAGGCTAT
AAGTAGAGTCCAACATCCAAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCANAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11