Sequences from confirmation amplicon


Confirmation sequences for line 676G12 and hit At1g60940 are shown.
Line ID 676G12
Locus At1g60940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22440839
Primers used for PCR AC11 - 8474, show primer details
   
Sequence Name 35-K027791-022-676-G12C-8409
GenBank Accession LN501697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K027791-022-676-G12C-8409
GCCATTTACAATTGAATAACCAAAATCACAGATCTTCAGGCGTGGAGCACGACTTCCATC
TAAGAGGGTATTTTCAAGCTTCAGATCTCTGTGGCATATTTGCTGTGACAATAAGGAACT
AATGCAAGTAAAGGACTGATACCGCAGAAATTATGTATATCTGTGAAGTTAAAAAAACTT
CGTAACCCATTTCTTTCTCTTACCATAGCATGACAATAGCTGACTCCTGAGATAAGCTGC
TGGAAAAAGTATCTTGCCTGAAAACAAAGATGTGCACAAAAAGCTATCATAATCGGAGAA
GCTGCTCCAAATGATTTACAACACATATTCCTTTTGCTTCCTGTACTACAAAACACAAAT
ATAATTCTACAAGATTTCCGCTTTCCTTTCCACTCAATTAATCTCAGACCACAATACAAC
TCTGAAATTGATCATTTAGTACACCACCATGACTTAAAATCTTTAAATGCCAGGGGAAGC
TCACCTCATCCTCACTGAATCTTCCACGCATACA
   
Sequence Name 36-K027791-022-676-G12C-AC11
GenBank Accession LN502089 [GenBank]
Sequencing primer gene specific primer, AC11: 5'-ATATGTAGCGCTGGAAGATTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K027791-022-676-G12C-AC11
GGCATTTAAGATTTTAAGTCATGGGTGGTGTACTAAATGATCAATTTCAGAGTTGTATTG
TGGTCTGAGATTAATTGAGTGGAAAGGAAAGCGGAAATCTTGTAGAATTATATTTGTGTT
TTGTAGTACAGGAAGCAAAAGGAATATGTGTTGTAAATCATTTGGAGCAGCTTCTCCGAT
TATGATAGCTTTTTGTGCACATCTTTGTTTTCAGGCAAGATACTTTTTCCAGCAGCTTAT
CTCAGGAGTCAGCTATTGTCATGCTATGGTAAGAGAAAGAAATGGGTTACGAAGTTTTTT
TAACTTCACAGATATACATAATTTCTGCGGTATCAGTCCTTTACTTGCATTAGTTCCTTA
TTGTCACAGCAAATATGCCACAGAGATCTGAAGCTTGAAAATACCCTCTTAGATGGAAGT
CCTGCTCCACGCCTGAAGATCTGTGATTTTGGTTATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11