SimpleSearch - Line and FST details


Line specific information

 
Line ID 676G12
Vector Used pAC161
Line Availability available as T3 set from NASC (N464884)
Segregation Analysis 50:50:34
Confirmed for Hit At1g60940
Parent of DUPLO pair none
Parent of pair(s) 92576, 92584, 92590, 92596, 92601, 92605, 92608, 92610, 92611

Gene hit T4D2

 
Sequence (A. th genome BLAST matches underlined) 
>95-K023062-022-676-G12-8409
NNTTTCGAGCAATTCCGGACATGAGCCATTTACATTGAATATATCCTGATGGTCATCTCA
CATGACGTGACTCGATCGAGTCCAATTCGTACTAATAATGCTCATCGAAACTATGCACTA
TTTTCTTTTCTATTTTGGCGGTTTAGGGAGGGCGATATGTTTAATTGGGTAGTAAATTAT
AATTTGAATATGAAGACGAGAAACCAACATCTTTTTCCATTCGATTCATTAAATATTCAA
ATGCATATATATATTAAAAGGAAAAGCAAAAGGATAATAGATTGTGGAATACTGCTGGAA
TTCAATGACCGTATGGGATCCTCCCGATACAGAGGATTTACAACACATATTCCTTTTGCT
TCCCGTATGGGATCCTCCCTATAGTGAGNNNNNNNNNNNNNNNNNNNNN
GenBank Accession BX661588 [GenBank]
Graphic View Graphic view of sequence BX661588 of line 676G12
Predicted Position of Insertion Chr3:19730289 - go to primer design
BLAST e Value 2e-118
Hit Clone Code (BAC ID) T4D2
Insertion Classification Genome Hit
Confirmation Status unknown
Other FSTs Supporting this Hit BX661588 [GenBank]

Gene hit At1g60940

 
Sequence (A. th genome BLAST matches underlined) 
>95-K022971-022-676-G12-8409
CATTTAAATTATATATATCACTGAATTATTTGGCCAATTGCGAGAAAATAATATATCTGT
ATCACCTTTCCATGGAATACTCCCATATAACTAGGTATGGGATCCTCCCTATAGTGAGAA
CTAATGCAAGTAAAGGACTGATACCGCAGAAATTATGTATATCTGTGAAGTTAAAAAAAC
TTCGTAACCCATTTCTTTCTCTTACCATAGCATGACAATAGCTGACTCCTGAGATAAGCT
GCTGGAAAAAGTATCTTGCCTGAAAACAAAGATGTGCACAAAAAGCTATCATAATCGGAG
AAGCTGCTCCAAATGATTTACAACACATATTCCTTTTGCTTCCTGTATGGGATCCTCCCT
ATAGTGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNN
GenBank Accession BX661586 [GenBank]
Graphic View Graphic view of gene At1g60940
Predicted Position of Insertion Chr1:22440855 - go to primer design
BLAST e Value 8e-115
Hit Clone Code (BAC ID) T7P1
Hit Gene Code At1g60940 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation SNF1-related protein kinase 2.10
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37