Sequences from confirmation amplicon


Confirmation sequences for line 679F03 and hit At2g01220 are shown.
Line ID 679F03
Locus At2g01220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:125522
Primers used for PCR 36uj - 8474, show primer details
   
Sequence Name 36-K117356-0022-679-F03C-36uj
GenBank Accession LN502382 [GenBank]
Sequencing primer gene specific primer, 36uj: 5'-GTCTTTTGACTCTTCGCTGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117356-0022-679-F03C-36uj
GCAGAGTAGTAATTGATAATATCTTTCGTTGTGGCCACATGTTGTGACTGTATGAATTGA
TTCCGTTGTTGGATTTTGGAGAGGCCTCTGGCATTTTGACAATTTTACTATCTCCAAACA
ACAAATCAGATAACTGTTCTTTTTCTCTGGAAAATAAAATAGAAACAATCTCACAAGCAG
ATTTGTTGATGCAGTGTTTGTGGGGGCGGCTACCCATGTTTTGAAATATCCGCAATAAAT
GCCGACAAACCACCGCTTACAATTGCACAGATTAAAGATCGTGTCAAGCAATTTGAAGTA
GTTGGTAGGCCGGATCTTTATTTTTCACTATCTATTTATCTTTTCAATCTTGTGCACCAC
TTTTTTGGTATCTCTATGATCCAATTGTTTCCAACTAGAGAAAGACCAATCAGTTTTATG
ATCCAGAGTCGAGCTATAATTGTAAATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 16-K117355-0022-679-F03C-8409
GenBank Accession LN493605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117355-0022-679-F03C-8409
ATTTCATTGAATAATCCTGATTTACAATTATAGCTCGACTCTGGATCATAAAACTGATTG
GTCTTTCTCTAGTTGGAAACAATTGGATCATAGAGATACCAAAAAAGTGGTGCACAAGAT
TGAAAAGATAAATAGATAGTGAAAAATAAAGATCCGGCCTACCAACTACTTCAAATTGCT
TGACACGATCTTTAATCTGTGCAATTGTAAGCGGTGGTTTGTCGGCATTTATTGCGGATA
TTTCAAAACATGGGTAGCCGCCCCCACAAACACTGCATCAACAAATCTGCTTGTGAGATT
GTTTCTATTTTATTTTCCAGAGAAAAAGAACAGTTATCTGATTTGTTGTTTGGAGATAGT
AAAATTGTCAAAATGCCAGAGGCCTCTCCAAAATCCAACAACGGAATCAATTCATACAGT
CACAACATGTGGCCACAACGAAAGATATTATCAATTACTACTCTTGCATAATATAGCATA
TCAGATTTAACTAAGAAACATAGAGACAGCGAAGAGTCAAAAGAC

Last Updated on 06.12.2018 16:11