Sequences from confirmation amplicon


Confirmation sequences for line 679F10 and hit At5g37350 are shown.
Line ID 679F10
Locus At5g37350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14793549
Primers used for PCR 39ve - 8474, show primer details
   
Sequence Name 25-K117967-0022-679-F10C-39ve
GenBank Accession LN497649 [GenBank]
Sequencing primer gene specific primer, 39ve: 5'-CTTGATTGGCTAGACGGTAAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117967-0022-679-F10C-39ve
CTTTTCCTTCTGCTCGTCGTCCTAATGCTCACGGTGGACATGGTTCACGCCCTAACTCTA
GTGCTCTTCAGCCTATTTCAAATAAGGCCCAGAAGCTTACTAGTCACGTTCGTGCTTCTC
CTTTGGAGGTACCGTTTCTTATCGAATTTTTGTTTAGAATTTGAAAAAAGCTTCTGAATT
TCTTATAAAGATATGATCTTTACATTTGAAATAGCTTCTGAATTGCTCATAGTCTCTGCT
CAAATGAACATTGAGTTTATAGGGTTTAGGTTCAGGTAGGTTTTGTGTAGCTGGAAATGT
GATTTTGAGTTAATTGATGATGCAGGGATGGGAAGGAAAAAGGTTTATAAATGGCTTCAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGGTTATTAT
   
Sequence Name 09-K117966-0022-679-F10C-8409
GenBank Accession LN490384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117966-0022-679-F10C-8409
CCATTTACAATTGAATATGAAGCCATTTATAAACCTTTTTCCTTCCCATCCCTGCATCAT
CAATTAACTCAAAATCACATTTCCAGCTACACAAAACCTACCTGAACCTAAACCCTATAA
ACTCAATGTTCATTTGAGCAGAGACTATGAGCAATTCAGAAGCTATTTCAAATGTAAAGA
TCATATCTTTATAAGAAATTCAGAAGCTTTTTTCAAATTCTAAACAAAAATTCGATAAGA
AACGGTACCTCCAAAGGAGAAGCACGAACGTGACTAGTAAGCTTCTGGGCCTTATTTGAA
ATAGGCTGAAGAGCACTAGAGTTAGGGCGTGAACCATGTCCACCGTGAGCATTAGGACGA
CGAGCATGAAGAGAAAAGCCACCACCAATTAGCTCGTCATCGTCTTTACCGTCAGCCCAA
TCAAG

Last Updated on 06.12.2018 16:11