Sequences from confirmation amplicon


Confirmation sequences for line 679F11 and hit At5g49020 are shown.
Line ID 679F11
Locus At5g49020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19873801
Primers used for PCR 43uv - 8474, show primer details
   
Sequence Name 87-K117671-0022-679-F11C-43uv
GenBank Accession LN514799 [GenBank]
Sequencing primer gene specific primer, 43uv: 5'-AAAGCAAAATTGACCTGAGAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K117671-0022-679-F11C-43uv
GGCATCAAATGAAGTCTACAGTGATCTCTTGCCCTGCCATGACGTGAATAGGCTGCGAGA
GAACACATCTTATTTGGTACCAATGGGTTGTTGGTGCACCGGGAGCAGTTGTAAACCATC
TTTGCACCGTGCTGTTGGATACAAAGCATCAGGTTTGTATGATATACCTTTGAGGCTTTG
ATGGTCAAAAAGGAATATTAATCTTTAGCTAGCACAGGATGAAAACATATAAATTGTTAC
GGCTTGATACCTCCCGTCAAAGAGAACATCAAACCAGCACGCAAGTCCATGAATTCTGGT
ACACACAGACGCAGTGAACTTCAACGGGATATCAATCTCATAAAATTGTTCTTCCTGTGA
AAAAAATTTGACAGTTATTGAATTTGGATCATGCCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 39-K117670-0022-679-F11C-8409
GenBank Accession LN503501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117670-0022-679-F11C-8409
ATCGGGCATGATCCAATTCATAACTGTCAAATTTTTTTCACAGGAAGAACAATTTTATGA
GATTGATATCCCGTTGAAGTTCACTGCGTCTGTGTGTACCAGAATTCATGGACTTGCGTG
CTGGTTTGATGTTCTCTTTGACGGGAGGTATCAAGCCGTAACAATTTATATGTTTTCATC
CTGTGCTAGCTAAAGATTAATATTCCTTTTTGACCATCAAAGCCTCAAAGGTATATCATA
CAAACCTGATGCTTTGTATCCAACAGCACGGTGCAAAGATGGTTTACAACTGCTCCCGGT
GCACCAACAACCCATTGGTACCAAATAAGATGTGTTCTCTCGCAGCCTATTCACGTCATG
GCAGGGCAAGAGATCACTGGTAGACTTCATTTGATTGCCCACAGTGCTCAGAGTTACACT
ATAAATCTAACACTCTCAGGTCAATTTTGCTTT

Last Updated on 06.12.2018 16:11