Sequences from confirmation amplicon


Confirmation sequences for line 681G05 and hit At1g26798 are shown.
Line ID 681G05
Locus At1g26798 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9282109
Primers used for PCR CQ77 - 8474, show primer details
   
Sequence Name 28-K107131-0022-681-G05C-CQ77
GenBank Accession LN498870 [GenBank]
Sequencing primer gene specific primer, CQ77: 5'-AAGAGCTTGATTGGTATGCAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107131-0022-681-G05C-CQ77
AAAACTGTGATCAATGATAAAACATAAAAATAACACGTGGTTATTTAATTTTTCTTGCAT
GTTTTGAAATTATAACCAAACTAAACAAACTTAGCTTTATTCATCTATCTAATACTAGTT
TTTTGGCATTTGGAAAAAAAAAACATTTCTCTTCAATTTAAAAGTGTCGTTATTCTATTG
ATTCCGATATTTCACAATTGGTCTATTCATCTGTTAATATGATATACCATTCAATCTCTA
AAATGTCCATGAAAAACAGAAAGGAGCCCCTCCATCGCGTCTAGCACGACATGGACCTGT
CTCACAAATATACCAAATACATTCTCTACATATTCCACATGTACTTTTTGAATTATCGTC
TCTATCTACTCTAAAAATATCAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCC
   
Sequence Name 12-K107130-0022-681-G05C-8409
GenBank Accession LN491602 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107130-0022-681-G05C-8409
TTACTTTGATATTTTTAGAGTAGATAGAGACGATAATTCAAAAAGTACATGTGGAATATG
TAGAGAATGTATTTGGTATATTTGTGAGACAGGTCCATGTCGTGCTAGACGCGATGGAGG
GGCTCCTTTCTGTTTTTCATGGACATCTTAGAGATTGAATGGTATATCATATTAACAGAT
GAATAGACCAATTGTGAAATATCGGAATCAATAGAATAACGACACTTTTAAATTGAAGAG
AAATGTTTTTTTTTTCCAAATGCCAAAAAACTAGTATTAGATAGATGAATAAAGCTAAGT
TTGTTTAGTTTGGTTATAATTTCAAAACATGCAAGAAAAATTAAATAACCACGTGTTATT
TTTATGTTTTATCATTGATCACAGTTTTATGAATAATAACTAAGTACTCATCAAAATTGT
TGAAAACGTGAAAAAACTGCATAC

Last Updated on 06.12.2018 16:11