Sequences from confirmation amplicon


Confirmation sequences for line 681G12 and hit At1g14600 are shown.
Line ID 681G12
Locus At1g14600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5002186
Primers used for PCR 52sm - 8474, show primer details
   
Sequence Name 39-K116518-0022-681-G12C-52sm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 52sm: 5'-CCAGAATCACTCTTCTTGGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116518-0022-681-G12C-52sm
TTTGGAGAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATGTCCC
C
   
Sequence Name 13-K116517-0022-681-G12C-8409
GenBank Accession LN492209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116517-0022-681-G12C-8409
GCCTTTCATTGAATTATCCTGATAACATACCTATTTTCTGGAATAAAATTAAAACACTTG
TCAGAAACAAATTAATATTCCCTTTCCGTTTTTTGCCAAGGAAATAATTTTTTTTTTTAC
CTAAAGTTAAAAGAGTGAAAACCCAAAAGACAATCATTCCTTGCGTGTACAGACAAGTTG
TCATGAAAATGATCTTCTTCATTGTCTTGCCTTCTTCTTCTTCTTGAAGAGGGGGAAAAA
CTCTCTTCTGTCCCCCAAAAAATATATTATAAAAACATTAATTAATTTATGACCATGATG
AATATTCTCTTCACTCTTTGGTAGGAGTATTTTTGTTTTTGGCAATAAATTAAAAGAAGC
ACTTATTTACCTATAAGTCTATAACCAAATTCTTTTTCATTTTTTTTGGTAAACCCCCCA
ACCCTTGTTTAAAAAAAAACTTTACATTATTTGAATATTTCGGACAAGGGGGGGAGGGAA
ATTTCAATTTATGCATTATTACAAATTTTATGTGAAGGGGGGGTATATAAAAAAAAATGT
CCTAATCTTGAAACTATGACGTTTGAGAGGGTAATTCGTTGATGCCCCCCCATATAAACA
ACAAAATATGTAAAACTCATTACCGGGTTTTCCAAAAAAAGTGATTCGGGA

Last Updated on 06.12.2018 16:11