Sequences from confirmation amplicon


Confirmation sequences for line 683D05 and hit At3g18090 are shown.
Line ID 683D05
Locus At3g18090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6200155
Primers used for PCR TH37 - 8474, show primer details
   
Sequence Name 55-K103582-0022-683-D05C-TH37
GenBank Accession LN508784 [GenBank]
Sequencing primer gene specific primer, TH37: 5'-CTAGAGGATACACAGAATGGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103582-0022-683-D05C-TH37
CTGTGAGCCTTGTGATTATGTATGATTCGTTGAACCTTTCTTAGATCATTGTTGATTGCC
AATGGTAAATATTTGTGGTTAGGGCCGGCTTTTCAAGATGGGGAAACGAAAGGGTCTACA
ATGGTAGATCGGGTGAGATGATGCGCTCTCTGATATTCATGGGCCCAACTTTCTACCAGC
GACTTGTCCACATGTCAGAGAACAAAGTCAAGTTCAGGAACACCGGACCAGTCCACCCGC
TCACACGCCAGCCAGTCGCAGACCGGAAGAGGTTTGGCGGGATAAGATTTGGAGAAATGG
AGCGAGACTGCCTAATAGCTCACGGTGCATCCGCTAATCTGCACGAGCGGCTCTTCACTC
TAAGTGACTCTTCTCAGATGCACATCTGCAGAAAATGCAAGACCTATGCGAATGTGATCG
AGAGGACTCCAAGCAGTGGAAGAAAGATCAGAGGGCCATATTGCAGAGTGTGCGCATCCT
CCGACCATGTGGTTAGGGTCTATGTTCCTTATGGAGCTAAGCTTCTTTGTCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 71-K103583-0022-683-D05C-8409
GenBank Accession LN512544 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K103583-0022-683-D05C-8409
TTGAATATATCCTGACAAGAAGCTTAGCTCCATAAGGAACATAGACCCTAACCACATGGT
CGGAGGATGCGCACACTCTGCAATATGGCCCTCTGATCTTTCTTCCACTGCTTGGAGTCC
TCTCGATCACATTCGCATAGGTCTTGCATTTTCTGCAGATGTGCATCTGAGAAGAGTCAC
TTAGAGTGAAGAGCCGCTCGTGCAGATTAGCGGATGCACCGTGAGCTATTAGGCAGTCTC
GCTCCATTTCTCCAAATCTTATCCCGCCAAACCTCTTCCGGTCTGCGACTGGCTGGCGTG
TGAGCGGGTGGACTGGTCCGGTGTTCCTGAACTTGACTTTGTTCTCTGACATGTGGACAA
GTCGCTGGTAGAAAGTTGGGCCCATGAATATCAGAGAGCGCATCATCTCACCCGATCTAC
CATTGTAGACCCTTTCGTTTCCCCATCTTGAAAAGCCGGCCCTAACCACAAATATTTACC
ATTGGCAATCAACAATGATCTAAGAAAGGTTCAACGAATCATACATAATCACAAGGCTCA
CAGCATCAATCCTCTAACTAATTATATACTGATACATGGCTTTTGAGCCCATTC

Last Updated on 06.12.2018 16:11