Sequences from confirmation amplicon


Confirmation sequences for line 684D08 and hit At3g46490 are shown.
Line ID 684D08
Locus At3g46490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17119259
Primers used for PCR JV34 - 8474, show primer details
   
Sequence Name 31-K101325-0022-684-D08C-8409
GenBank Accession LN500091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101325-0022-684-D08C-8409
CCATTTACATTGAATAATCCTGCCAGTCTGAAAACAACCTTCCCCATTAAGCATTTAACA
CTTCACCTTGTTACATTAAGACCTTCAACTAGTAACATTGCCTTATAGATGTTTATGGTT
GTGTTAAATGAATAGATATCCAGCGATCAAATGTTCAACGTACATCTCCCAACGTTACAA
AGCAGTACATGCACACCTAAAACAGACCTAGGTAGGTGAAGTATGGTCCATGCGTACCAC
GCTCTAAAGTCCCACGATATGAATGCTTCGTTTTCCAATTGGTTTGTTGTTAAAATTCTA
TATCTATCTGATGGAGATCTATTCAATTAATAAAGATCATCATTTAAGTGATTACAGATG
AATTTCTCGAATCGGAATTTTGGGTTATTTATTTCAGTTTACAAAATCATGGAAGACGCT
ACGTCCTCTATTTACGACGGTTATTTGGTATTTTAATATAAATTTTGGGGGAAAAAATCT
GTTTGAGATGTTTTGGAATGATTATGTGAATTATGTCCAAGTTTTGTAATCACAAGC
   
Sequence Name 31-K101331-0022-684-D08C-JV34
GenBank Accession LN500092 [GenBank]
Sequencing primer gene specific primer, JV34: 5'-TGCTTGTGATTACAAAACTTGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101331-0022-684-D08C-JV34
TTCAAACTCTCAACAGATTTTTTCCCCCAAAATTTATATTAAAATACCAAATAACCGTCG
TAAATAGAGGACGTAGCGTCTTCCATGATTTTGTAAACTGAAATAAATAACCCAAAATTC
CGATTCGAGAAATTCATCTGTAATCACTTAAATGATGATCTTTATTAATTGAATAGATCT
CCATCAGATAGATATAGAATTTTAACAACAAACCAATTGGAAAACGAAGCATTCATATCG
TGGGACTTTAGAGCGTGGTACGCATGGACCATACTTCACCTACCTAGGTCTGTTTTAGGT
GTGCATGTACTGCTTTGTAACGTTGGGAGATGTACGTTGAACATTTGATCGCTGGATATC
TATTCATTTAACACAACCATAAACATCTATNAGGCAATGTTACTAGTTGAAGGTCTTAAT
GTAACAAGGTGAAGTGTTAAATGCTTACTTGGGAAGGTTGTTTTCAGACTGGCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGAT
GTCCGCA

Last Updated on 06.12.2018 16:11