Sequences from confirmation amplicon


Confirmation sequences for line 685C03 and hit At2g03520 are shown.
Line ID 685C03
Locus At2g03520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1070425
Primers used for PCR 20ou - 8474, show primer details
   
Sequence Name 67-K115121-0022-685-C03C-20ou
GenBank Accession LN511875 [GenBank]
Sequencing primer gene specific primer, 20ou: 5'-CGTCATTGGTATACTTCCTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K115121-0022-685-C03C-20ou
GTTTCTTGCGTATCACGGTTGAATTTATAGGTACAACATTGAATTATTTCTTGGACAACA
AGATAAACAAAGCCGAGATCCTCTTCCCTGGCGTAGGTTGCTTCTTGATCGCTGTTTTCC
TAGGCGCTGCGGTTCATGCTTCTAATGCAGCTGATGTCAAAGAGAAACTCAAGTCTTTAC
CTAGCGAGTATAAAGCTGAGACAAACTAAGATGTTGCTCTTTCTTTTGCCTTTGTTCCAT
GTTTTAAAAATGTTTTAACAAAACTCTGATCTTTTTCAGAGATTTATATTCTTCTATTGA
AAATGGTGAAGACAAGCCTGAAATAGGTAAAACCTATATATATATTGATTTGTGTGAAGA
TATATGCAAAGCAAAGGAATGAAAATTCAGTTAAAATTAGAAACTTTTGGTGGAACTTTG
ATCAAAGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCC
   
Sequence Name 19-K115120-0022-685-C03C-8409
GenBank Accession LN494883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115120-0022-685-C03C-8409
ACTTTGATCAAAGTTCCACCAAAAGTTTCTAATTTTAACTGAATTTTCATTCCTTTGCTT
TGCATATATCTTCACACAAATCAATATATATATAGGTTTTACCTATTTCAGGCTTGTCTT
CACCATTTTCAATAGAAGAATATAAATCTCTGAAAAAGATCAGAGTTTTGTTAAAACATT
TTTAAAACATGGAACAAAGGCAAAAGAAAGAGCAACATCTTAGTTTGTCTCAGCTTTATA
CTCGCTAGGTAAAGACTTGAGTTTCTCTTTGACATCAGCTGCATTAGAAGCATGAACCGC
AGCGCCTAGGAAAACAGCGATCAAGAAGCAACCTACGCCAGGGAAGAGGATCTCGGCTTT
GTTTATCTTGTTGTCCAAGAAATAATTCAATGTTGTACCTATAAAATTCAACCGTGATAC
GCAAGAAACCATAGAAACATAAGTTTTTGATTCATCACAAACCAAAGGAAGTAACCAAAT
GACG

Last Updated on 06.12.2018 16:11